Beagle犬卵母细胞体外成熟研究进展

发布时间：2018-02-26 18:21

本文关键词： 犬疾病模型卵母细胞体外成熟　出处：《中国实验动物学报》2017年01期 　论文类型：期刊论文

【摘要】：国内实验Beagle犬种质资源保存利用及制备基因修饰人类疾病动物模型,要求有充足的Beagle犬胚胎。目前诱导排卵技术在犬上效果不明显,体内获取犬成熟卵母细胞有困难。同时,尽管科研人员对犬卵母细胞体外成熟培养进行了多方面的尝试,但尚未获得突破,成熟率低,严重制约了其在种质资源保存、基因修饰模型制备及生物医学研究中的应用。本文梳理了不同犬龄及生殖周期、卵母细胞形态与体积及脂滴在Beagle犬卵母细胞体外成熟中的影响,以为其体外成熟寻找新的突破口。
[Abstract]:Preservation and utilization of experimental Beagle dog germplasm resources and preparation of genetically modified animal models of human disease require sufficient Beagle dog embryos. At present, the technique of ovulation induction is not effective in dogs, so it is difficult to obtain mature canine oocytes in vivo. Although researchers have tried in many ways to mature canine oocytes in vitro, they have not made a breakthrough and have a low rate of maturation, which seriously restricts the preservation of canine oocytes in germplasm resources. The effects of age, reproductive cycle, oocyte morphology and volume and lipid droplets on the maturation of Beagle canine oocytes in vitro were studied. For its in vitro maturation to find a new breakthrough.
【作者单位】：广州医药研究总院有限公司实验动物研究开发中心国家犬类实验动物种子中心;
【基金】：广州市“珠江科技新星”项目(201610010144) 广州市科技基础条件平台建设项目(201605040005)
【分类号】：R-332

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