体外人外周血单个核细胞热原检测法用于ACYW135群脑膜炎球菌多糖疫苗的方法学研究

发布时间：2018-02-27 19:19

本文关键词： 体外人外周血单个核细胞热原检测法 ACYW群脑膜炎球菌多糖疫苗　出处：《科技导报》2017年17期 　论文类型：期刊论文

【摘要】：为考查体外人外周血单个核细胞热原检测法对ACYW135群脑膜炎球菌多糖疫苗的适用性,选取不同厂家共10批供试品,使用体外人外周血单个核细胞热原检测法,采用加样回收干扰实验考察供试品是否存在干扰。结果表明,大部分供试品在最大有效稀释倍数内,IL-6法干扰实验的回收率在50%~200%,且热原物质含量均低于标准限值,均为合格样品。研究表明,体外人外周血单个核细胞热原检测法可用于ACYW135群脑膜炎球菌多糖疫苗热原检测。
[Abstract]:In order to investigate the applicability of human peripheral blood mononuclear cell pyrogen assay to ACYW135 group meningococcal polysaccharide vaccine in vitro, ten batches of samples from different manufacturers were selected, and human peripheral blood mononuclear cell pyrogen assay was used in vitro. The results showed that the recovery rate of most of the samples was 50 ~ 200 and the content of pyrogen was lower than the standard limit. The results showed that pyrogen assay of human peripheral blood mononuclear cells could be used for pyrogen detection of ACYW135 meningococcal polysaccharide vaccine in vitro.
【作者单位】：中国食品药品检定研究院药理室;
【基金】：国家“重大新药创制”科技重大专项(2012ZX09304010)
【分类号】：R392-33

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