五羟黄酮对博莱霉素诱导大鼠肺纤维化模型的预防及治疗作用

发布时间：2018-02-28 18:34

  本文关键词： 五羟黄酮 肺纤维化 博莱霉素　出处：《广东医学》2017年15期 　论文类型：期刊论文

【摘要】：目的探讨五羟黄酮对博莱霉素(BLM)诱导大鼠肺纤维化模型的预防及治疗作用。方法将雄性C57BL/6小鼠随机分为假手术组、BLM模型组(80 mg/kg)、五羟黄酮小剂量组(40 mg/kg)、五羟黄酮大剂量组(80mg/kg)、地塞米松(DXM)组,造模后第10天,静脉给药20 d,于造模后第30天处死动物、取材。通过病理学检查及肺组织羟脯氨酸含量确定肺纤维损伤及程度;测定肺泡支气管灌洗液(BALF)蛋白含量反映炎性细胞因子的释放;测定致炎性细胞因子肿瘤坏死因子-α(TNF-α)在BALF的水平及在肺组织的表达,以及促纤维化细胞因子血小板源性生长因子(PDGF-BB)和转化生长因子β1(TGF-β1)在肺组织的表达,以探讨五羟黄酮防治肺纤维化的可能机制。结果与BLM模型组相比,五羟黄酮40及80 mg/kg均明显缓解实验第7及30天的炎症细胞浸润、胶原纤维形成等病理学改变,显著降低纤维化面积,并降低实验第30天羟脯氨酸含量。五羟黄酮大剂量组还可降低实验第7及30天BLM引起的BALF中蛋白含量的升高。对于致炎性细胞因子TNF-α,在实验第7及30天,五羟黄酮小、大剂量组BALF中其含量及肺组织中其表达水平均较BLM模型组低;对于促纤维化细胞因子TGF-β1和PDGF-BB,与BLM模型组相比,五羟黄酮40及80 mg/kg显著降低实验第30天肺组织中两者的表达。结论五羟黄酮对BLM诱导的肺纤维化模型具有预防及治疗作用,可能与其下调肺组织TNF-α、TGF-β1和PDGF-BB的表达有关。
[Abstract]:Objective to investigate the preventive and therapeutic effects of pentaflavone on bleomycin induced pulmonary fibrosis in rats. Methods male C57BL / 6 mice were randomly divided into sham-operation group (n = 80 mg / kg), low dose group (n = 40 mg / kg) and pentaflavone group (n = 40 mg / kg). The dose group was 80 mg / kg, and the dexamethasone DXM group, On the 10th day, 20 days after intravenous administration, the animals were killed on the 30th day, and the lung fiber injury and degree were determined by pathological examination and the content of hydroxyproline in lung tissue. The content of BALF- protein in bronchoalveolar lavage fluid (BALF) was determined to reflect the release of inflammatory cytokines, the expression of TNF- 伪 in BALF and the expression of TNF- 伪 in lung tissue were measured. And the expression of PDGF-BBand TGF- 尾 _ 1 in lung tissue to explore the possible mechanism of pentaflavone in the prevention and treatment of pulmonary fibrosis. Results compared with BLM model group, the expression of PDGF-BBB and TGF- 尾 _ 1 in lung tissue was studied. At 40 and 80 mg/kg, pentaflavone significantly alleviated the inflammatory cell infiltration, collagen fiber formation and other pathological changes on the 7th and 30th days of the experiment, and significantly reduced the area of fibrosis. It also decreased the content of hydroxyproline on the 30th day of the experiment. The increase of protein content in BALF induced by BLM on the 7th and 30th day was also decreased in the high-dose group. For the inflammatory cytokine TNF- 伪, on the 7th and 30th days, the pentaflavone was small. The content of BALF and the expression of TGF- 尾 1 and PDGF-BBB in BALF and lung tissue in high dose group were lower than those in BLM model group, and compared with BLM model group, the levels of TGF- 尾 1 and PDGF-BBs in high dose group were lower than those in BLM model group. Conclusion Pentaflavone can prevent and treat pulmonary fibrosis induced by BLM, which may be related to its down-regulation of the expression of TNF- 伪 -TGF- 尾 1 and PDGF-BB in lung tissue.
【作者单位】： 黄河科技学院医学院;郑州大学第一附属医院肝胆外科;
【基金】：河南省医学教育研究课题(编号:wjlx2015197)
【分类号】：R285.5;R-332
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本文编号：1548459