鲍曼不动杆菌外排泵基因与菌株多药耐药关系的研究

发布时间：2018-03-01 18:41

本文关键词： 鲍曼不动杆菌外排泵基因耐药性 PCR扩增　出处：《中国病原生物学杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的分析鲍曼不动杆菌耐药情况及外排泵基因分布情况,为临床感染治疗及耐药性控制提供指导。方法收集本院各科室送检的患者样本,对菌株进行分离培养,经全自动细菌鉴定仪鉴定出鲍曼不动杆菌110株,采用K-B法对菌株耐药性进行分析,用PCR扩增法对菌株外排泵基因分布情况进行检测。结果 110株鲍曼不动杆菌中分离自ICU 41株,呼吸内科25株,神经外科15株,心脏外科12株,肿瘤科9株以及其他科室8株,分别占37.27%、22.73%、13.64%、10.91%、8.18%和7.27%。各科室鲍曼不动杆菌的分离率分别为40.20%、25.77%、23.81%、21.05%、16.36%、和7.77%。自痰液标本75株,导管标本12株,血液标本8株,尿液标本6株,引流液标本4株,其他标本5株,分别占68.18%、10.91%、7.27%、5.45%、3.64%和4.55%;不同标本的分离率分别为38.66%、15.58%、15.09%、13.04%、9.52%和7.69%。鲍曼不动杆菌对环丙沙星、诺氟沙星、卡那霉素、利福平、四环素、红霉素、亚胺培南和美罗培南等常用抗菌药物的耐药率分别为50.91%、49.09%、63.64%、37.27%、77.27%、81.82%、20.91%和26.36%。adeA基因+adeB基因、adeB基因+adeC基因、adeA基因+adeB基因+adeC基因、mdfA基因+adeA基因+adeB基因+adeC基因、qacE△1基因+adeA基因+adeB基因+adeC基因、mdfA基因+qacE△1基因+adeA基因+adeB基因+adeC基因的检测率分别为1.82%、3.64%、44.55%、25.45%、10.91%和8.18%。结论鲍曼不动杆菌对常用抗菌药物均产生了不同程度的耐药性,可能与菌株携带外排泵基因有关。
[Abstract]:Objective to analyze the drug resistance of Acinetobacter baumannii and the distribution of efflux pump gene in order to provide guidance for clinical infection treatment and drug resistance control. One hundred and ten strains of Acinetobacter baumannii were identified by automatic bacterial identification instrument. The drug resistance of the strains was analyzed by K-B method. The gene distribution of efflux pump was detected by PCR amplification. Results 41 strains of ICU were isolated from 110 strains of Acinetobacter baumannii. There were 25 strains of respiratory medicine, 15 strains of neurosurgery, 12 strains of cardiac surgery, 9 strains of oncology and 8 strains of other departments, accounting for 37.27% and 22.7313.64%, 10.91% and 7.27%, respectively. The isolation rates of Acinetobacter baumannii were 40.2025.7723.811.05% 16.3636, and 7.777.75 sputum specimens, 12 catheterized specimens, 12 catheterized specimens, respectively. There were 8 blood samples, 6 urine specimens, 4 drainage fluid specimens, and 5 other specimens, accounting for 68.18% 10.91 and 7.27% 5.45%, 3.64% and 4.55%, respectively. The isolation rates of the different specimens were 38.66, 15.5815.09, 15.09, 13.049.52% and 7.696.Acinetobacter baumannii against ciprofloxacin, norfloxacin, kanamycin, rifampicin, and rifampicin, respectively, were used in the treatment of ciprofloxacin, norfloxacin, kanamycin and rifampicin. Tetracycline, erythromycin, The drug resistance rates of imipenem and meropenem were 50.91and 49.0933.64, respectively. 77.272.The resistance rates of imipenem and meropenem were 20.91% and 26.36%, respectively. The resistance rates of imipenem and meropenem were 20.91% and 26.36%, respectively. The resistance rates of common antimicrobial agents such as imipenem and meropenem were 50.91% and 26.36%, respectively. The resistance rates of common antimicrobial agents such as imipenem and meropenem were 49.09 and 63.64, respectively. The resistance rates of imipenem and meropenem were 20.91. AdeA gene, adeB gene, adeC gene, qacE 1 gene, adeA gene, adeB gene, adeC gene were 1.82% and 3.64%, respectively. Conclusion Acinetobacter baumannii has different drug resistance to common antimicrobial agents. It may be related to the strain carrying efflux pump gene.
【作者单位】：宁波市医疗中心李惠利医院重症医学科;宁波市李惠利医院东部医院检验科;
【分类号】：R378

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