几种细胞脂肪变性模型的建立与比较分析

发布时间：2018-03-02 05:21

本文关键词： 降脂减肥诱导脂肪变性模型细胞　出处：《中华中医药学刊》2017年08期 　论文类型：期刊论文

【摘要】：高脂血症和肥胖已成为严重的公共健康问题,引起了人们的广泛关注。脂肪变性细胞模型是研究降脂减肥的重要手段之一。比较分析了诱导人肝L-02细胞、人肝HepG2细胞、小鼠3T3-L1前脂肪细胞、大鼠肝BRL细胞、人肝Changliver细胞、奶牛肝细胞、草鱼肝细胞(L8824)等不同细胞株建立高脂细胞模型的方法和效果。诱导多在细胞对数生长末期,并多采用脂肪酸、脂肪乳、高浓度胎牛血清等诱导培养24~48 h。不同细胞株及不同的诱导方法,细胞中甘油三酯(TG)和总胆固醇(TC)的升高幅度有较大差异。
[Abstract]:Hyperlipidemia and obesity have become a serious public health problem, which has attracted wide attention. Fatty degeneration cell model is one of the most important methods to reduce fat and weight loss. Human liver L-02 cells and human liver HepG2 cells induced by hyperlipidemia and obesity have been compared and analyzed. The methods and effects of establishing high fat cell models by mouse 3T3-L1 preadipocytes, rat liver BRL cells, human liver Changliver cells, cow hepatocytes and grass carp hepatocytes L8824) were established. Adipose milk and high concentration fetal bovine serum were cultured for 24 h for 48 h. Different cell lines and different induction methods showed significant difference in the increase of TGG and TCC in the cells.
【作者单位】：云南中医学院中药学院;昆明理工大学生命科学与技术学院;
【基金】：国家自然科学基金项目(21466037) 国家科技支撑计划项目(2011BAD46B00) 云南省教育厅科学研究重大专项项目(ZD2014009) 云南省应用基础研究-中医联合专项项目(U0120150242)
【分类号】：R-332;R589.2

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