赤芍总苷对肺癌模型大鼠抑癌相关基因表达的影响

发布时间：2018-03-02 16:43

本文选题：赤芍总苷　切入点：肺癌　出处：《中国药房》2016年16期 　论文类型：期刊论文

【摘要】：目的:研究赤芍总苷对肺癌模型大鼠抑癌相关基因表达的影响。方法:将90只大鼠随机分为正常组、模型组、阳性对照组[环磷酰胺,50 mg/(kg·d)]和赤芍总苷低、中、高剂量组[50、100、200 mg/(kg·d)],每组15只。除正常组外,其余各组大鼠均采用左肺叶支气管内一次性灌注致癌碘油液复制肺癌模型,造模成功后,每周周一至周五各组大鼠于背iv相应药物,正常组和模型组iv等体积生理盐水,连续16周。采用逆转录-聚合酶链反应法测定大鼠肺组织中多药耐药相关蛋白(MRP)、人多药耐药基因(MDR1)和凋亡相关基因P21、P16 m RNA的表达;采用免疫组化法测定肺组织中抑癌基因P53蛋白的表达,计算其阳性率;观察肺组织病理变化。结果:与正常组比较,模型组大鼠肺组织中MRP、MDR1、P21、P16 m RNA及P53蛋白表达(阳性率为66.67%)水平明显升高(P0.05);与模型组比较,阳性对照组和赤芍总苷低、中、高剂量组大鼠肺组织中MRP、MDR1、P21、P16 m RNA及P53蛋白表达(阳性率分别为46.67%、46.67%、40.00%、13.33%)水平降低并呈剂量依赖性,且赤芍总苷中、高剂量组比阳性对照组降低程度更显著(P0.05);赤芍总苷各剂量组间比较,各指标差异均有统计学意义(P0.05)。结论:赤芍总苷可明显抑制肺癌模型大鼠MRP、MDR1、P21、P16基因及P53蛋白的表达。
[Abstract]:Objective: to study the effect of total glucosides of paeony on the expression of tumor suppressor genes in lung cancer model rats. Methods: 90 rats were randomly divided into normal group, model group, positive control group [cyclophosphamide 50 mg/(kg 路d] and total glucoside of paeony. High dose group [50,100,200 mg/(kg 路d], 15 rats in each group. Except the normal group, all the other groups were treated with one-off instillation of carcinogenic lipiodol fluid into the left lobar bronchus to make lung cancer model. After the successful establishment of the model, the rats in each group were given corresponding drugs in the back from Monday to Friday every week. The expression of multidrug resistance-associated protein (MDR1), human multidrug resistance gene (MDR1) and apoptosis-related gene P21p16 m RNA were detected by reverse transcription-polymerase chain reaction (RT-PCR) in normal group and model group iv for 16 weeks. The expression of tumor suppressor gene p53 protein in lung tissue was determined by immunohistochemical method, the positive rate was calculated, and the pathological changes of lung tissue were observed. The expression of P16m RNA and p53 protein (positive rate was 66.67) in the lung tissue of the model group was significantly higher than that in the model group, and the total glucosides in the positive control group and paeony root group were lower than those in the model group. The expression of MRPMDR1P16m RNA and p53 protein in lung tissue of high dose group was decreased in a dose-dependent manner (the positive rates were 46.67 ~ 46.67 ~ 46.67g ~ 40.005 ~ 13.33, respectively). In the total glucosides of paeony, the level of P0.05in the high dose group was significantly lower than that in the positive control group, and there was a significant difference among the different dose groups of the total glucoside of paeony, and the expression of P16 RNA and p53 protein in the lung tissue of the high dose group was significantly lower than that of the positive control group. Conclusion: total glucoside of paeony can significantly inhibit the expression of P16 gene and p53 protein in lung cancer model rats.
【作者单位】：承德医学院附属医院肿瘤科;承德医学院附属医院病理科;
【分类号】：R285.5;R-332

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