成簇的规律间隔短回文重复序列在致病菌中分型研究及应用

发布时间：2018-03-03 06:38

本文选题：成簇规律间隔短回文重复序列　切入点：分子分型　出处：《生物技术》2017年02期 　论文类型：期刊论文

【摘要】：为全面深入了解CRISPR在致病菌中的最新进展,该文简介了CRISPR的结构与功能,深入论述其在各类致病菌中的分型研究及应用情况,剖析CRISPR分型研究现有问题和措施,并展望今后CRISPR重点研究方向。CRISPR分型在各类致病菌中有着不同程度应用,在结核分枝杆菌复合群、沙门氏菌、产志贺毒素大肠杆菌等致病菌中已获成功。加强多种致病菌的全基因组测序及扩充CRISPR数据库,CRISPR分型将会应用更广泛。
[Abstract]:In order to understand the latest progress of CRISPR in pathogenic bacteria, the structure and function of CRISPR were briefly introduced, the research and application of CRISPR in all kinds of pathogenic bacteria were discussed, and the existing problems and measures of CRISPR typing were analyzed. It also looks forward to the application of CRISPR typing in various pathogenic bacteria in the future, especially in Mycobacterium tuberculosis complex, Salmonella, Salmonella, Salmonella, Salmonella, Mycobacterium tuberculosis, Salmonella, Salmonella, and Salmonella. Shigella toxin-producing Escherichia coli and other pathogenic bacteria have been successfully used. It will be more widely used to strengthen the whole genome sequencing of a variety of pathogenic bacteria and to expand the CRISPR database of CRISPR typing.
【作者单位】：张家港出入境检验检疫局综合技术中心;
【基金】：国家重点研发计划项目(“跨境食品潜在、新发病原微生物筛查监控技术及溯源平台建设”,No.2016YFD0401102) 江苏出入境检验检疫局青年基金项目(“特殊位点序列分析在食源性致病菌中的研究及应用”,No.2016KJ49;“弯曲杆菌高通量同步检测分型技术研究及检测试剂盒AOAC认证”,No.2016KJ53) 质检总局科研项目(“食品中弯曲杆菌多重实时荧光PCR检测分型技术研究及应用”,No.2016IK308)
【分类号】：Q78;R37

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