建立大鼠高肺血流性肺动脉高压模型的简易方法

发布时间：2018-03-03 21:22

本文选题：肺动脉高压　切入点：高肺血流　出处：《广州医科大学学报》2016年05期 　论文类型：期刊论文

【摘要】：目的:简化、改良建立大鼠高肺血流性肺动脉高压模型的方法。方法:采用腹主动脉-下腔静脉穿刺分流术建立大鼠高肺血流性肺动脉高压模型,并以组织黏合剂闭合腹主动脉左侧穿刺口。分流11周后,观察下腔静脉变化情况,通过右心导管术检测大鼠肺动脉压力,以血气分析方法测定动静脉血氧饱和度,分离右心室与左心室(包含室间隔)并计算右心室肥厚指数。结果:腹主动脉阻断时间少于2 min;11周后分流组大鼠下腔静脉明显增粗并存在震颤及动静脉血混合,其分流瘘口之上的下腔静脉血氧饱和度约等于腹主动脉血氧饱和度,但明显高于假手术组下腔静脉血氧饱和度(P0.01),分流组大鼠平均肺动脉压及右心室肥厚指数显著高于假手术组(P0.01)。结论:采用组织黏合剂的方法能简易、快速地完成腹主动脉-下腔静脉穿刺分流术,有效地建立大鼠高肺血流性肺动脉高压模型。
[Abstract]:Objective: to simplify and improve the method of establishing a rat model of pulmonary hypertension with high pulmonary blood flow. Methods: a rat model of pulmonary hypertension with high pulmonary blood flow was established by abdominal aorta-inferior vena cava shunt. After 11 weeks of shunt, the changes of inferior vena cava were observed, pulmonary artery pressure was measured by right cardiac catheterization, and arterial and venous oxygen saturation was measured by blood gas analysis. The right ventricle and left ventricle (including septum) were separated and the right ventricular hypertrophy index was calculated. Results: the abdominal aorta occlusion time was less than 2 mins and 11 weeks later, the inferior vena cava was thickened and there was tremor and mixed arteriovenous blood in the shunt group. The oxygen saturation of the inferior vena cava above the shunt fistula is about equal to that of the abdominal aorta. The mean pulmonary artery pressure and the right ventricular hypertrophy index in shunt group were significantly higher than those in sham operation group. Conclusion: the method of tissue adhesive can be used as a simple method. Abdominal aorta-inferior vena cava shunt (IVC) was performed quickly to establish a rat model of pulmonary hypertension with high pulmonary blood flow.
【作者单位】：广州医科大学附属广州市妇女儿童医疗中心心脏中心;
【基金】：国家自然科学基金(81100233)
【分类号】：R544.1;R-332

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