蕨麻猪与巴马小型猪脏器指数及低氧相关基因的比较

发布时间：2018-03-04 12:04

本文选题：蕨麻猪　切入点：巴马小型猪　出处：《中国比较医学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的研究蕨麻猪与巴马小型猪脏器指数及低氧相关基因表达量的差异。方法采用Taq Man探针实时定量PCR(real-time quantitative PCR)技术,检测蕨麻猪与巴马小型猪心、肝、脾、肺、肾低氧反应基因表达量的变化。结果蕨麻猪的肾脏、脾脏指数低于巴马小型猪,蕨麻猪的心脏、肺脏指数高于巴马小型猪。蕨麻猪血管内皮细胞生长因子(Vascular endothelial growth factor,VEGF)与低氧诱导因子-1α(hypoxia inducible factor 1,HIF-1α)在肺、肾中的表达量明显比普通猪高(P0.05或P0.01);促红细胞生成素(erythropoietin,EPO)只在肺中比巴马小型猪高(P0.05)。结论不同物种脏器指数可能是物种对物质环境、能量环境、外界病原体、能量代谢需求等综合因素进化适应的结果。蕨麻猪低氧相关基因明显比巴马小型猪高,揭示在基因方面蕨麻猪具有较强的高原适应性。
[Abstract]:Objective to study the difference of organ index and hypoxia related gene expression between fern hemp pig and Bama miniature pig. Methods using Taq Man probe, real-time PCR(real-time quantitative PCR technique was used to detect heart, liver, spleen and lung of fern hemp pig and Bama miniature pig. Results the indexes of kidney and spleen of fern hemp pigs were lower than those of Bama miniature pigs and fern hemp pigs. Pulmonary index was higher than that of Bama miniature pig. Vascular endothelial growth factor (VEGF) and hypoxia inducible factor 1 (HIF-1 伪) were found in the lung of fern hemp pig. The expression of erythropoietin in the kidney was significantly higher than that in the normal pigs (P0.05 or P0.01). Erythropoietin erythropoietin (EPO) was only higher in the lung than that in the Bama miniature pig (P 0.05). Conclusion the visceral index of different species may be the species' response to physical environment, energy environment and external pathogens. The results showed that Hypoxia-related genes in fern hemp pigs were significantly higher than those in Bama miniature pigs, which indicated that fern hemp pigs had strong genetic adaptability to plateau.
【作者单位】：兰州军区兰州总医院动物实验科;
【基金】：甘肃省自然科学基金项目(1308RJZA178)
【分类号】：R-33

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