腹腔注射LPS建立认知功能障碍相关的中枢神经系统免疫炎症小鼠模型

发布时间：2018-03-06 07:09

本文选题：认知功能障碍　切入点：脂多糖　出处：《中国病理生理杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的:建立认知功能障碍相关的中枢神经系统免疫炎症小鼠模型。方法:实验采用9~11周的C57/6J雄性小鼠,腹腔注射脂多糖(lipopolysaccharide,LPS)500μg/kg或750μg/kg。采用Morris水迷宫和避暗实验评价小鼠的认知功能,爬杆试验检测小鼠的运动协调性。免疫荧光法观察小鼠海马区神经元特异性微管相关蛋白2(microtubule-associated protein 2,MAP-2)的表达和小胶质细胞的形态和数量。Western blot实验检测小鼠脑组织匀浆液中环氧合酶2(cyclo-oxygenase-2,COX-2)和诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)蛋白的表达。结果:Morris水迷宫结果提示,与对照组相比,LPS组小鼠逃避潜伏期延长,目标象限停留时间及穿越目标象限次数减少(P0.05);避暗实验结果提示LPS组均能使小鼠的潜伏期缩短,错误次数增加(P0.05);爬杆实验结果提示LPS组小鼠爬完全程时间高于正常对照组;LPS组小鼠海马区的神经元减少,小胶质细胞增加,COX-2和i NOS蛋白表达增加(P0.01)。结论:腹腔注射LPS可引起小鼠认知功能障碍,模拟认知功能障碍相关的中枢炎症动物模型。
[Abstract]:Objective: to establish a mouse model of central nervous system immune inflammation associated with cognitive dysfunction. Methods: C57 / 6J male mice were injected intraperitoneally with lipopolysaccharide 500 渭 g / kg or 750 渭 g / kg. The Morris water maze and avoidance experiments were used to evaluate the cognitive function of the mice. The expression of neuron specific microtubule-associated protein 2 (2) and microtubule-associated protein 2 (MAP-2) and the morphology and number of microglia in mouse brain were detected by immunofluorescence assay. Western blot assay was used to detect the homogenate of mouse brain tissue. The expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) protein in the solution. Compared with the control group, the escape latency of lipopolysaccharide group was prolonged, the retention time of target quadrant and the times of crossing target quadrant were decreased (P 0.05), the results of avoidance experiment indicated that LPS group could shorten the latency of mice. The number of errors increased (P 0.05), and the results of pole climbing test showed that the whole course time of the LPS group was higher than that of the normal control group, and the neurons in the hippocampal area of the control group were decreased. Conclusion: intraperitoneal injection of LPS can induce cognitive dysfunction in mice and simulate the central inflammatory model associated with cognitive dysfunction.
【作者单位】：暨南大学附属第一医院;暨南大学医学院病理生理学系国家中药管理局三级科研实验室;
【基金】：国家自然科学基金资助项目(No.81371442) 广东省高等学校优秀青年教师培养计划(No.YQ2015024) 广州市科技计划珠江科技新星专项(No.201506010095)
【分类号】：R-332;R741

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