2013～2014年我国输入性B3基因型麻疹病毒的基因特征分析

发布时间：2018-03-06 15:19

本文选题：麻疹病毒　切入点：病毒学监测　出处：《病毒学报》2017年04期 　论文类型：期刊论文

【摘要】：研究2013~2014年中国大陆分离到的输入性B3基因型麻疹野毒株的N蛋白羧基端核苷酸和氨基酸特征。应用MEGA6.0软件对2013~2014年输入我国的B3基因型麻疹病毒、GenBank下载的WHO参考株、2013~2014年全球流行的B3基因型麻疹病毒代表株和中国疫苗株泸191(S191)构建基于N蛋白COOH端450个核苷酸片段序列的亲缘性关系树、分析其核苷酸和氨基酸差异。13株B3基因型中国分离株间的核苷酸序列和氨基酸序列同源性分别为99.7%~100%和100%;与B3基因型WHO参考株的核苷酸和氨基酸同源性分别为96.6%~96.8%和95.3%~97.3%;与2013~2014年流行的B3基因型的麻疹野病毒代表株的核苷酸和氨基酸同源性分别在90.0%~100%和87.9%~100%;与S191的核苷酸和氨基酸同源性分别为93.3%~93.5%和87.2%。本研究对积累我国麻疹病毒分子流行病学基线数据具有很重要的意义,随着我国进入麻疹消除加速阶段,将会监测到更多的非本土基因型的输入,需要进一步加强病毒学监测,防止输入性麻疹野病毒在我国扩散和传播。
[Abstract]:The characteristics of N-protein carboxyl terminal nucleotides and amino acids of imported wild type B 3 measles virus isolated from mainland China from 2013 to 2014 were studied. The WHO reference strain downloaded from GenBank of genotype B 3 virus imported into China from 2003 to 2014 was analyzed by MEGA6.0 software. A genetic relationship tree based on 450 nucleotide fragment sequences of N protein COOH terminal was constructed from the representative strain of measles virus of genotype B3 and the Chinese vaccine strain Lu191S191 from 2003 to 2014. The homology of nucleotide sequence and amino acid sequence among Chinese isolates were 99.777% and 100%, respectively, and the homology of nucleotide and amino acid with B3 genotype WHO reference strain were 96.66% and 96.8%, respectively. The nucleotide and amino acid homology of the wild measles virus representative strain of B3 genotype from 2013 to 2014 were 90.010% and 87.9%, respectively, and the nucleotide and amino acid homology with S191 were 93.33% and 87.2%, respectively. Epidemiological baseline data are of great significance, As China enters the accelerated stage of measles elimination more non-native genotypes will be detected and virological surveillance should be further strengthened to prevent the spread and transmission of imported wild measles viruses in China.
【作者单位】：中国疾病预防控制中心病毒病预防控制所卫生部医学病毒学和病毒病重点实验室世界卫生组织西太平洋区麻疹参比实验室;烟台市疾病预防控制中心;安徽理工大学医学院;
【基金】：国家自然科学基金课题(项目号:81371791),题目:麻疹病毒血凝素蛋白分子进化抗原变异及疫苗保护效果的影响~~
【分类号】：R373.11

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