β-胡萝卜素对脂多糖刺激的IPEC-J2细胞紧密连接蛋白表达的影响

发布时间：2018-03-07 22:28

本文选题：β-胡萝卜素　切入点：IPEC-J细胞　出处：《中国免疫学杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的:为研究β-胡萝卜素对肠上皮细胞的抗炎作用机制。方法:选用仔猪空肠上皮(IPEC-J2)细胞系建立体外细胞模型,将同一代数的IPEC-J2细胞分为4组(空白对照组、β-胡萝卜素组、β-胡萝卜素预保护组和脂多糖组)。空白对照组不做处理,β-胡萝卜素组和β-胡萝卜素预保护组均进行β-胡萝卜素预处理,脂多糖(LPS)组和β-胡萝卜素预保护组均采用LPS刺激。MTT法检测细胞活力;Western blot法检测Occludin、Claudin4、ZO-1紧密连接蛋白表达水平。结果:LPS组IPEC-J2细胞的紧密连接蛋白表达水平均低于空白对照组,且差异有统计学意义(P0.05),β-胡萝卜素预保护组紧密连接蛋白表达水平显著高于LPS组(P0.05)。结论:提高紧密连接蛋白表达水平可能是β-胡萝卜素对空肠上皮细胞的抗炎作用的途径之一。
[Abstract]:Objective: to study the anti-inflammatory mechanism of 尾 -carotene on intestinal epithelial cells. The IPEC-J2 cells of the same algebra were divided into 4 groups (blank control group, 尾 -carotene group, 尾 -carotene preprotection group and lipopolysaccharide group). The blank control group was not treated, the 尾 -carotene group and 尾 -carotene preprotection group were pretreated with 尾 -carotene. Lipopolysaccharide (LPS) group and 尾 -carotene preprotection group were used to detect the activity of IPEC-J2 cells by LPS stimulation. Western blot assay was used to detect the expression of close-junction protein in IPEC-J2 cells. Results the expression of tight junction protein in IPEC-J2 cells was lower than that in control group. The expression of tight junction protein in 尾 -carotene preprotection group was significantly higher than that in LPS group (P 0.05). Conclusion: increasing the expression level of tight junction protein may be one of the ways of 尾 -carotene anti-inflammatory effect on jejunal epithelial cells.
【作者单位】：吉林农业大学动物科学技术学院;
【基金】：国家自然基金项目资助项目(31672511)
【分类号】：R392

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