电针委中穴对腰椎退变模型大鼠纤维环细胞caspase3、XIAP蛋白表达的影响

发布时间：2018-03-08 06:12

本文选题：委中穴　切入点：椎间盘退变　出处：《中国康复医学杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的:观察电针委中穴对腰椎退变模型大鼠纤维环细胞凋亡及caspase3和XIAP表达的影响。方法:将40只雄性SD大鼠按随机数字表法分为假手术组、模型组、电针委中穴组、电针非穴组。采用纤维环穿刺法制作腰椎退变模型。于造模1个月后行电针治疗,电针委中穴组取"委中"穴,电针非穴组取委中内侧0.5cm,均每日1次,每次20min,连续治疗4周。治疗结束后取材,运用TUNEL法检测纤维环细胞凋亡,免疫组化检测(caspase3)和XIAP表达。结果:假手术组偶见TUNEL细胞,caspase3低表达,XIAP高表达;模型组纤维环细胞TUENL阳性细胞和caspase3表达较假手术组显著增加(P0.01),XIAP表达低于假手术组(P0.01);与模型组比较,电针委中穴组TUNEL阳性细胞和caspase3表达均明显减少(P0.01),XIAP表达增加(P0.01);与电针委中穴组比较,电针非穴组纤维环细胞TUENL阳性细胞和caspase3表达较高(P0.05),XIAP蛋白表达减少(P0.05)。结论:电针可延缓椎间盘退变,其机制可能与调节纤维环细胞caspase3和XIAP表达,抑制纤维环细胞凋亡有关。
[Abstract]:Objective: to observe the effect of electroacupuncture (EA) on apoptosis and expression of caspase3 and XIAP in fibrocytic cells of lumbar degenerative rats. Methods: forty male Sprague-Dawley rats were randomly divided into sham-operation group, model group and EA Weizhong point group. The model of lumbar degenerative degeneration was made by using fiber loop puncture method. Electroacupuncture treatment was performed 1 month later. The acupoints of "Wei Zhong" in the acupoint group and 0.5 cm on the medial side in the non-point group were obtained once a day. After 20 min treatment for 4 weeks, the apoptosis of fibrous ring cells was detected by TUNEL method, and the expression of caspase3) and XIAP were detected by immunohistochemistry. Results: in sham-operation group, the low expression of TUNEL cell caspase3 and the high expression of XIAP were occasionally observed. The expression of TUENL positive cells and caspase3 in the model group was significantly higher than that in the sham-operated group, and the expression of P0.01XIAP in the model group was lower than that in the sham-operated group. The positive cells and caspase3 expression of TUNEL were significantly decreased in the group of acupoints in the electroacupuncture group, and the expression of XIAP was increased in the group of P0.01, which was significantly lower than that in the group of acupoints in the electroacupuncture group. The expression of TUENL and caspase3 was higher in the non-acupoint group than in the control group. Conclusion: electroacupuncture can delay the degeneration of intervertebral disc, which may be related to the regulation of caspase3 and XIAP expression and the inhibition of apoptosis.
【作者单位】：福建中医药大学;福建中医药大学附属康复医院;
【基金】：国家自然科学基金资助项目(81303017) 福建省自然科学基金(2014J01346) 福建省教育厅A类科技项目(JA13163)
【分类号】：R246.9;R-332

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