2,4,6-三硝基苯磺酸和葡聚糖硫酸钠诱导的大鼠肠炎模型比较

发布时间：2018-03-08 13:23

本文选题：-三硝基苯磺酸　切入点：葡聚糖硫酸钠　出处：《中国药房》2017年10期 　论文类型：期刊论文

【摘要】：目的:比较2,4,6-三硝基苯磺酸(TNBS)和葡聚糖硫酸钠(DSS)诱导的两种肠炎大鼠模型。方法:SD大鼠随机分为正常对照组、TNBS模型组和DSS模型组,每组10只。TNBS模型组大鼠采用TNBS 50%乙醇溶液以100 mg/kg一次性灌肠法建立肠炎模型,DSS模型组大鼠采用4%DSS水溶液连续7 d自由饮用建立肠炎模型。观察各组大鼠一般情况,检测血清总胆固醇(TC)和三酰甘油(TG)含量、结肠长度及质量、结肠组织中髓过氧化物酶(MPO)活性,肉眼观察结肠大体外观形态,苏木精-伊红染色观察结肠病理学变化。结果:与正常对照组比较,TNBS模型组大鼠体质量明显减轻,结肠长度明显缩短、单位长度质量明显增加,粪便性状评分、血清TG含量、MPO活性、结肠大体外观评分和病理学评分均明显增加(P0.05或P0.01);DSS模型组大鼠体质量明显减轻,结肠长度明显缩短,粪便性状和结肠病理学评分均明显增加(P0.05或P0.01)。与DSS模型组比较,TNBS模型组大鼠结肠长度明显缩短、单位长度质量明显增加,血清TC、TG含量和结肠大体外观评分、病理学评分明显增加(P0.05或P0.01),组织病理学检查可见明显肠道溃疡与粘连。结论:DSS诱导肠炎模型大鼠粪便评分较TNBS诱导模型低,TNBS诱导肠炎模型大鼠在肠道组织损伤方面较DSS诱导模型更严重、恢复更慢。
[Abstract]:Objective: to compare two kinds of enteritis rat models induced by TNBS) and sodium dextran sulfate (DSS). Methods: the rats were randomly divided into two groups: normal control group and DSS model group. TNBS 50% ethanol solution was used to establish enteritis model rats in each group of 10 rats. The rats in the model group were fed with 4DSS aqueous solution for 7 days to establish enteritis model. The general situation of the rats in each group was observed. The contents of total cholesterol (TC) and triglyceride (TG), the length and quality of colon, the activity of myeloperoxidase (MPO) in colon tissue, and the gross appearance of colon were observed with naked eyes. Results: compared with the normal control group, the body weight, colon length, unit length and fecal traits of TNBS model rats were significantly reduced, and the weight of the unit length was significantly increased, compared with the normal control group, the pathological changes of colon were observed by hematoxylin and eosin staining. Serum TG content and MPO activity, colonic gross appearance score and pathological score were significantly increased in DSS model group (P0.05 or P0.01). Compared with the DSS model group, the colonic length, the weight per unit length, the serum TCTG content and the colonic gross appearance score were significantly increased in the DSS model group. Histopathological examination showed obvious intestinal ulcer and adhesion. Conclusion the stool score of rats with TNBS induced enteritis is lower than that of TNBS-induced enteritis model rats. Surface is more serious than DSS model. Recovery is slower.
【作者单位】：浙江中医药大学附属杭州市第一人民医院临床药理学研究室;浙江中医药大学附属杭州市第一人民医院转化医学研究中心临床药理学研究室;
【分类号】：R-332;R574

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