TRPA1在母婴分离大鼠的内脏高敏感发生中的作用

发布时间：2018-03-09 12:11

本文选题：母婴分离　切入点：内脏高敏感　出处：《福建医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：采用母婴分离大鼠，观察大鼠背根神经节中瞬时感受器电位锚蛋白-1(TRPA1)表达变化，及其与内脏感觉敏感性、脊髓背角降钙素基因相关肽(CGRP)和P物质(SP)表达水平的关系，探讨TRPA1在母婴分离大鼠内脏高敏感性发生中的作用。方法：按析因设计，将32只新生雄性SD大鼠随机分为4组，每组8只。A1B1组：正常饲养+结直肠扩张(CRD)前腹腔注射生理盐水；A1B2组：正常饲养+CRD前腹腔注射钌红；A2B1组：母婴分离+CRD前腹腔注射生理盐水；A2B2组，母婴分离+CRD前腹腔注射钌红。大鼠成年后进行CRD来观察腹壁撤退反射(AWR)和腹外斜肌肌电(EEOA)的检测，取远端结肠组织进行HE检测，并用免疫组化观察L6-S1节段背根神经节中的TRPA1和相应脊髓背角中CGRP、SP的表达。结果： (1)母婴分离可以使大鼠受CRD刺激时AWR评分升高，，母婴分离对AWR评分影响的主效应在CRD压力40~80mmHg时，均有统计学意义（p值分别为0.036、0.009、0.027）。CRD前腹腔注射钌红可以降低AWR评分，钌红对AWR评分影响的主效应在CRD压力40~60mmHg时，有统计学意义（p值分别为0.036、0.039）。CRD压力20~80mmHg时母婴分离和钌红对AWR评分的影响无显著性交互作用(p值分别为0.361、0.128、0.352、0.130)； (2)母婴分离可以使大鼠在受CRD刺激时EEOA值升高，主效应在CRD压力为40~80mmHg时，均有统计学意义（p值分别为0.000、0.000、0.000）。CRD前腹腔注射钌红可降低EEOA值，主效应在60~80mmHg时，均有统计学意义（p值分别为0.003、0.039）。CRD压力20~80mmHg时母婴分离和钌红对EEOA值的影响无显著性交互作用(p值分别为0.744、0.931、0.062、0.572)； (3)母婴分离可以使TRPA1和CGRP、SP的平均光密度值升高，其主效应均有统计学意义（p值分别为0.001、0.000、0.000）。CRD前腹腔注射钌红对TRPA1和CGRP、SP的平均光密度值影响的主效应均无统计学意义（p值分别为0.186、0.509、0.579）。母婴分离和钌红对TRPA1和CGRP、SP的影响均无显著性交互作用(p值分别0.321、0.936、0.777)； (4)TRPA1与CGRP、SP、AWR评分、EEOA值之间分别存在正相关关系（p均0.05）。结论：新生期母婴分离可引起大鼠内脏高敏感性，背根神经节TRPAl参与内脏高敏感性的形成。
[Abstract]:Objective: to observe the changes of transient receptor potential anchor protein (-1TRPA1) expression in rat dorsal root ganglion (DRG) and its relationship with visceral sensibility, calcitonin gene-related peptide (CGRPP) and substance P substance (SP) expression in dorsal horn of rats. To investigate the role of TRPA1 in the development of visceral hypersensitivity in rats with maternal and infant separation. Methods: according to the factorial design, 32 newborn male SD rats were randomly divided into 4 groups. Eight rats in each group were treated by intraperitoneal injection of saline A1B2 before normal colorectal dilatation. Group A 2B1 was injected intraperitoneally before normal feeding of CRD: group A 2B2 was injected with normal saline before CRD. Ruthenium red was injected intraperitoneally before mother-to-child separation (CRD). CRD was used to observe the abdominal wall withdrawal reflex (CRD) and the external oblique muscle electromyography (EEOAA), and the distal colon tissue was taken for HE detection. The expression of TRPA1 in L6-S1 segment dorsal root ganglion and the expression of CGRP PSP in the corresponding dorsal horn of spinal cord were observed by immunohistochemistry. Results:. 1) Maternal and infant separation could increase the AWR score of rats stimulated by CRD. The main effect of maternal and infant separation on AWR score was 0.036 卤0.009 ~ 0.027 卤0.027. The main effect of maternal and infant separation on AWR score was 0.036 卤0.009 ~ 0.027 mm Hg, respectively. The AWR score could be decreased by intraperitoneal injection of ruthenium red. The main effect of ruthenium red on AWR score was 0.036 卤0.039 卤0.039 mm Hg for CRD pressure and 0.036 卤0.039 mm Hg for CRD pressure, respectively, and there was no significant interaction between Ru red and Ru Red on AWR score (P = 0.361) 0.1280.3520.130 mm Hg, respectively. (2) Maternal and infant separation could increase the EEOA value of rats stimulated by CRD, and the main effect was that when the CRD pressure was 40,80mmHg, there was significant difference in the main effect between the two groups. The value of Ru red could be decreased by intraperitoneal injection of ruthenium red before CRD, and the main effect was at 60 ~ 80mmHg. There was no significant interaction between maternal and infant separation and ruthenium red on EEOA value at the pressure of 20 ~ 80mmHg (P = 0.003 ~ 0.039), P = 0.744 ~ (0.931) ~ (0.062) ~ (2) ~ (0.572g), P = 0.74 4 ~ (-1) ~ 0. 931 ~ 0. 062 卤0. 572 mm Hg, respectively. 3) the average optical density of TRPA1 and CGRP SP was increased by maternal separation. There was no significant difference in the main effects of Ru red on the average optical density of TRPA1 and CGRP SP before the injection of ruthenium red. The main effects were not statistically significant. The effects of maternal and infant separation and ruthenium red on TRPA1 and CGRPSP were not significant, respectively. There was no significant difference in the effects of Ru red on TRPA1 and CGRPSP before CRD. The main effects of Ru on TRPA1 and CGRPSP were not statistically significant (P = 0. 186, P = 0. 509 / 0. 579, respectively). The P value of significant interaction was 0. 321 卤0. 936 and 0. 777m, respectively. There was a positive correlation between TRPA1 and SPAWR score and EEOA value. Conclusion: neonatal maternal and infant separation can induce visceral hypersensitivity in rats. TRPAl in dorsal root ganglion is involved in the formation of visceral hypersensitivity.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363

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