调衡方多糖增强体外培养巨噬细胞的吞噬能力

发布时间：2018-03-09 20:36

本文选题：调衡方多糖　切入点：巨噬细胞　出处：《细胞与分子免疫学杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的研究调衡方多糖对巨噬细胞吞噬功能的影响,探讨其多糖对巨噬细胞吞噬作用的免疫调节机制。方法依照中药多糖的制备方法从调衡方中提取粗多糖;从小鼠腹腔分离提取并培养巨噬细胞;(500、200、10)μg/m L调衡方多糖处理巨噬细胞48 h,光学显微镜下观察活细胞的形态,墨汁吞噬实验以及金黄色葡萄球菌吞噬实验观察巨噬细胞的吞噬能力,酶细胞化学染色观察巨噬细胞胞内酸性磷酸酶的变化。结果与对照组比较,(500、200、10)μg/m L调衡方多糖处理巨噬细胞后,巨噬细胞体积显著增大,对墨汁和金黄色葡萄球菌的吞噬能力均显著提高,酸性磷酸酶的活性也明显增强,并与多糖浓度呈正相关。结论调衡方多糖能增强巨噬细胞的吞噬功能,并提高细胞内酸性磷酸酶的活性。
[Abstract]:Objective to study the effect of Tiao-heng recipe polysaccharide on phagocytic function of macrophages and to explore the immunomodulatory mechanism of its polysaccharide on macrophage phagocytosis. Methods crude polysaccharides were extracted from Tiao-heng recipe according to the preparation method of traditional Chinese medicine polysaccharides. Macrophages were isolated and cultured from the abdominal cavity of mice. The macrophages were treated with the polysaccharide of Tiao-heng recipe for 48 h. The morphology of living cells, the phagocytosis of Chinese ink and the phagocytosis of Staphylococcus aureus were observed under optical microscope, and the phagocytic ability of macrophages was observed by the experiment of Staphylococcus aureus phagocytosis. The changes of intracellular acid phosphatase in macrophages were observed by enzyme cytochemical staining. Results compared with the control group, the volume of macrophages was significantly increased after treated with Tiaoheng recipe (500 渭 g / m ~ (-1) ~ (10)) 渭 g / m ~ (-1). The phagocytic ability of both Chinese ink and Staphylococcus aureus was significantly increased, and the activity of acid phosphatase was also significantly increased, which was positively correlated with the concentration of polysaccharides. Conclusion Tiaoheng polysaccharide can enhance the phagocytic function of macrophages. The activity of acid phosphatase in cells was also increased.
【作者单位】：陕西中医药大学;
【基金】：陕西省科技厅自然基础科研基金(2014JM4202) 陕西省中医管理局科研基金(13-JC022)
【分类号】：R392.12

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