复方黄栌口服液对小鼠黄疸型肝炎模型肝脏中指标的影响

发布时间：2018-03-10 13:01

本文选题：复方黄栌口服液　切入点：小鼠　出处：《中华中医药学刊》2017年08期 　论文类型：期刊论文

【摘要】：目的:探讨复方黄栌口服液对小鼠黄疸型肝炎模型肝脏指标的影响。方法:将72只KM小鼠随机分为空白组,模型组,护肝宁片组(1.012 5 g/kg),大、中、小剂量复方黄栌口服液组(30、15、7.5 mL/kg),每组12只,空白组和模型组灌胃相同体积的生理盐水,连续灌胃相应的药物10 d。各组于实验第8天,灌胃ANIT造模,48 h后取材。小鼠脱颈椎处死后,从肝右叶切取肝组织1块,10%中性福尔马林固定,脱水,包埋,切片,HE染色,观察组织病理学变化。另取肝脏,用冷生理盐水制匀浆,检测肝匀浆MDA、SOD、TC、TG。结果:与模型组比,大、中、小剂量复方黄栌口服液组均可显著降低肝脏TC、TG、MDA水平(P0.01),显著升高肝脏SOD水平(P0.01)。大、小剂量复方黄栌口服液组可显著降低小鼠肝指数(P0.01),中剂量复方黄栌口服液组可明显降低小鼠肝指数(P0.05)。结论:复方黄栌口服液组和护肝宁片组可明显减轻造模所致的小鼠肝损伤。
[Abstract]:Objective: to investigate the effect of compound Cotinus coggygria oral liquid on liver indexes in mice with jaundice hepatitis. Methods: 72 km mice were randomly divided into blank group, model group and Huganning tablet group (1.0125 g / kg). Small dose of Cotinus coggygria oral liquid group (n = 12 in each group) was given normal saline of the same volume in blank group and model group for 10 days. Each group was treated with ANIT for 48 h, and the mice were killed by cervical vertebra removal. A 10% neutral formalin was removed from the right lobe of the liver to immobilize, dehydrate, embed it, stained sections with HE, observed the histopathological changes. In addition, the liver homogenate was prepared with cold normal saline, and the liver homogenate TCCG was detected. Results: compared with the model group, the liver homogenate was larger and more moderate. The low dose compound Cotinus coggygria oral liquid group could significantly reduce the level of liver TCC TGG SOD and increase the liver SOD level (P 0.01). Low dose coggygria coggygria oral liquid group could significantly reduce liver index (P 0.01) of mice, while medium dose compound coggygria oral liquid group could significantly reduce liver index (P 0.05) of mice. Conclusion: Cotinus coggygria coggygria compound oral liquid group and Huganning tablet group can obviously reduce the liver injury induced by model making in mice.
【作者单位】：中国人民解放军第三七一中心医院;
【基金】：新乡重点科技攻关项目(ZG14012)
【分类号】：R285.5;R-332

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