EGCG对尿酸诱导人脐静脉内皮细胞炎症反应及氧化应激的影响

发布时间：2018-03-13 00:18

本文选题：表没食子儿茶素没食子酸酯　切入点：尿酸　出处：《山东医药》2017年12期 　论文类型：期刊论文

【摘要】：目的探讨表没食子儿茶素没食子酸酯(EGCG)对尿酸(UA)诱导人脐静脉内皮细胞(HUVEC)炎症反应和氧化应激的影响。方法体外培养HUVEC,取第4~5代细胞随机分为四组,对照组给予EGM-2完全培养液培养;UA组加入含终浓度为8 mg/d L UA的EGM-2完全培养液培养;UA+EGCG组加入含终浓度为50μmol/L EGCG的EGM-2完全培养液培养12 h,再加入含终浓度为8 mg/d L UA的EGM-2完全培养液培养;EGCG组加入含终浓度为50μmol/L EGCG的EGM-2完全培养液培养。收集各组培养24 h的细胞,采用RT-PCR法检测NF-κB、单核细胞趋化蛋白1(MCP-1)、细胞间黏附分子1(ICAM-1)、环氧化酶2(COX-2)、TNF-α、诱导型一氧化氮合酶(i NOS)mRNA相对表达量,采用DCFH-DA法检测活性氧(ROS)水平(以平均光密度值表示)。结果与对照组比较,UA组NF-κB、MCP-1、ICAM-1、TNF-α、i NOS mRNA相对表达量均升高(P均0.01);与UA组比较,UA+EGCG组、EGCG组NF-κB、MCP-1、ICAM-1、COX-2、TNF-α、i NOS mRNA相对表达量均下降(P均0.01)。对照组、UA组、UA+EGCG组、EGCG组平均光密度值分别为101.26±12.34、345.98±27.01、112.65±9.62、103.37±5.88,UA组高于对照组,UA+EGCG组、EGCG组均低于UA组,组间比较P均0.01。结论 EGCG可减轻UA诱导HUVEC发生的炎症和氧化应激反应,抑制NF-κB信号通路、减少炎症因子释放可能是其作用机制。
[Abstract]:Objective to investigate the effects of epigallocatechin gallate (EGCGG) on inflammatory response and oxidative stress induced by uric acid (UAA) in human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured in vitro, and the 4th generation of HUVECs were randomly divided into four groups. The control group was treated with EGM-2 complete culture medium for 12 h, and the EGM-2 complete culture medium containing 8 mg/d L UA at 8 mg/d L UA was added to the EGCG group for 12 hours. The EGM-2 complete culture medium containing 50 渭 mol/L EGCG was added for 12 h, and the final concentration was 8 mg/d L UA. EGM-2 cells were cultured in 50 渭 mol/L EGCG (50 渭 mol/L EGCG) EGM-2 culture medium for 24 h, and the cells in each group were cultured for 24 h. The relative expression of NF- 魏 B, monocyte chemoattractant protein (MCP-1), intercellular adhesion molecule (ICAM-1), cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) NOS)mRNA were detected by RT-PCR assay. DCFH-DA method was used to detect the level of reactive oxygen species (Ros) (expressed as the average optical density). Results compared with the control group, the relative expression of NF- 魏 B, MCP-1and ICAM-1TNF- 伪 NOS mRNA in UA group was significantly higher than that in UA group, and the relative expression of NF- 魏 BMCP-1COM-1COX-2TNF- 伪 NOS mRNA in UA EGCG group was significantly lower than that in UA group. The average optical density of UA EGCG group in control group was 101.26 卤12.34U 345.98 卤27.01U 112.65 卤9.62n 103.37 卤5.88U, respectively, which was significantly lower than that in UA EGCG group. Conclusion EGCG can attenuate the inflammation and oxidative stress of HUVEC induced by UA, inhibit NF- 魏 B signaling pathway, and decrease the release of inflammatory factors.
【作者单位】：复旦大学附属华东医院;
【基金】：国家自然科学基金资助项目(81100603)
【分类号】：R363

【相似文献】