束缚-应激联合辣素灌胃建立大鼠腹泻型肠易激综合征模型及评价

发布时间：2018-03-13 10:29

本文选题：腹泻型肠易激综合征　切入点：束缚-应激　出处：《中国比较医学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的基于观察大鼠症状学、组织病理学、内脏敏感性、肥大细胞活化、自噬、Beclin-1及Claudin-2等相关指标变化,评价束缚-应激联合辣素灌胃建立一种新的腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,D-IBS)模型研究。方法清洁级雄性SD大鼠40只,按体重分为正常组、模型I组、模型II组及模型III组,每组10只。模型组均采用束缚-夹尾应激联合辣素(I组0.125%、II组0.250%、III组0.500%)灌胃制备D-IBS模型,正常组予等剂量生理盐水灌胃处理,连续2周。分别应用Power lab仪检测大鼠腹壁收缩次数及弓背次数变化,肥大细胞染色液(醛品红-橙黄G法)检测肥大细胞活化情况,采用光镜、电镜检测大鼠结肠组织病理学及其自噬变化,应用免疫组化SABC法检测大鼠结肠黏膜Beclin-1及Claudin-2表达变化。结果模型III组大鼠全部死亡,模型II组大鼠大便次数增多,内脏敏感性阈值降低,与正常组及模型I组比较,差异有显著性(P0.05);各组大鼠结肠黏膜、黏膜上皮绒毛及腺体形态正常,未见黏膜下血管扩张及弥漫性炎症浸润改变;正常组除外,模型I组、II组大鼠结肠组织黏膜间质或黏膜下层可见类圆形紫红至深紫色染色点,提示肥大细胞表达增多;模型II组大鼠结肠肠上皮细胞自噬、Beclin-1及Claudin-2表达明显增多,与正常组及模型I组相比,差异有显著性(P0.05)。结论束缚-应激联合0.25%辣素灌胃建立的大鼠D-IBS模型以腹泻增多、内脏高敏感、肥大细胞表达增高、肠上皮细胞自噬增加、肠黏膜屏障破坏等表现为特点,与人IBS较为相似,该方法简单易行,值得推广应用。
[Abstract]:Objective to observe the changes of symptoms, histopathology, visceral sensitivity, mast cell activation, autophagy Beclin-1 and Claudin-2 in rats. To evaluate the effect of restraint stress combined with pepsin on the establishment of a new model of diarrhea irritable irritable bowel syndrome D-IBSs. Methods Forty clean male SD rats were divided into normal group, model group I, model group II and model III group according to their body weight. The D-IBS model was made by intragastric administration of restraint and tail stress combined with capsaicin I group (0.125%) and normal saline group (0.500%) in group II (group II) and normal saline group (n = 10), and normal control group (n = 10), and normal group (n = 10) were treated by intragastric administration of normal saline at the same dose. For 2 weeks, the changes of the number of contraction of abdominal wall and the number of times of arched back were detected by Power lab instrument, and the activation of mast cells in mast cell staining solution (aldehyde fuchsin orange G method) was detected by light microscope. The changes of colonic histopathology and autophagy were examined by electron microscope, and the expression of Beclin-1 and Claudin-2 in colonic mucosa of rats were detected by immunohistochemical SABC method. Results all the rats in model III group died and the defecation times in model group II increased. The visceral sensitivity threshold was significantly lower than that in normal group and model group I, the colonic mucosa, epithelial villi and glands were normal in each group, no submucosal vasodilation and diffuse inflammatory infiltration were observed. Except the normal group, the colonic mucosa stroma or submucosa of the model group I and II showed round purplish red to dark purple staining points, indicating the increase of mast cell expression. Compared with normal group and model I group, the expression of Beclin-1 and Claudin-2 in colonic epithelial cells of model II rats was significantly higher than that in normal group and model I group. Conclusion the D-IBS model of rats induced by restraint stress combined with 0.25% caprine is more diarrhea. The hypersensitivity of viscera, the increase of mast cell expression, the increase of intestinal epithelial cell autophagy and the destruction of intestinal mucosal barrier are characteristic features, which are similar to human IBS. This method is simple and easy to use and is worth popularizing.
【作者单位】：广西中医药大学附属瑞康医院消化科;广西中医药大学第一附属医院消化科;浙江省中西医结合医院消化科;
【基金】：国家自然科学基金(NO:81460724) 广西自然科学基金(NO:2014GXNSFAA118212)
【分类号】：R-332;R574.62

【相似文献】