自然衰老ICR小鼠围绝经期模型的建立与评价

发布时间：2018-03-14 20:31

本文选题：自然衰老　切入点：围绝经期　出处：《中华中医药杂志》2016年06期 　论文类型：期刊论文

【摘要】：目的:研究自然衰老ICR小鼠围绝经期模型的建立,并进行模型评价。方法:通过观察阴道脱落细胞,了解自然衰老小鼠及青年小鼠的动情周期情况;观察各组小鼠的自主活动,了解各组小鼠的情绪状态;比较血清卵泡刺激素(FSH)、促黄体激素(LH)、雌二醇(E_2)及孕酮(P)的含量;测定小鼠下丘脑神经肽,了解围绝经期下丘脑神经肽分泌的变化,比较下丘脑KNDy及相关受体m RNA的表达,判定小鼠进入围绝经期。结果:自然衰老组动情周期紊乱(P0.01),动情频率明显减少(P0.01),自主活动次数显著提高(P0.01),血清FSH、LH水平显著升高(P0.01),E_2、P水平明显降低(P0.01,P0.05),下丘脑KNDy及相关受体m RNA的表达明显升高(P0.01)。结论:自然衰老法能建立小鼠围绝经期模型。
[Abstract]:Objective: to study the establishment and evaluation of the peri-menopausal model of natural aging ICR mice. Methods: by observing vaginal exfoliated cells, the estrous cycle of natural aging mice and young mice was studied, and the autonomous activities of each group of mice were observed. To understand the emotional state of the mice in each group; to compare the contents of serum follicle stimulating hormone (FSH), luteinizing hormone (LHH), estradiol (E 2) and progesterone (P); to determine the hypothalamic neuropeptides in mice and to understand the changes of hypothalamic neuropeptide secretion during the menopausal period. To compare the expression of KNDy and m RNA in hypothalamus, Results: in natural aging group, the frequency of estrus decreased significantly, the frequency of estrus decreased significantly, the frequency of spontaneous activity increased significantly, the level of serum FSHN LH increased significantly, the level of P0.01P decreased significantly, the hypothalamus KNDy and related P0.05 decreased significantly. The expression of receptor m RNA increased significantly (P 0.01). Conclusion: the natural aging method can establish the model of mouse menopausal period.
【作者单位】：杭州市中医院;湖州市中心医院;萧山区第一人民医院;
【基金】：浙江省科技厅项目(No.2013C33211) 浙江省中医药管理局项目(No.2013ZQ025) 浙江省卫生厅项目(No.2013KYA168) 杭州市科技局项目(No.20140733Q31) 国家名老中医药专家傅萍传承工作室建设项目(No.[2014]20号)~~
【分类号】：R-332;R271.116

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