石膏铁丝固定法建立兔膝痹模型

发布时间：2018-03-17 04:16

本文选题：模型　切入点：动物　出处：《中国组织工程研究》2016年18期 　论文类型：期刊论文

【摘要】：背景:目前国内外均有采用石膏固定法复制膝痹动物模型,但存在实验动物选择不佳、石膏选取不合适、单纯石膏固定不稳定等问题,影响后续实验结果。目的:采用石膏铁丝固定法建立兔膝痹模型。方法:将20只新西兰大白兔随机均分为2组,模型组在应用树脂石膏屈曲位固定的基础上外层缠绕铁丝以保护石膏,建立右侧膝痹模型;对照组为正常对照。固定后8周,进行右膝关节大体观察与病理组织学观察。结果与结论:对照组膝关节半透明,光滑,有光泽,无软骨缺损,软骨弹性及硬度好,软骨细胞排列正常,关节囊内未见炎细胞浸润;模型组膝关节无光泽,不透明,关节软骨变薄,弹性较差,关节软骨退变,关节面变粗糙,部分有缺损,关节内仅有少量关节液或无关节液,软骨细胞萎缩或消失,数量减少,软骨变性、坏死,软骨下骨组织骨质硬化,骨小梁增生,软骨细胞紊乱无序,玻璃样变,滑膜绒毛肥大,细胞成簇现象,可见大量淋巴细胞、浆细胞、中性粒细胞浸润,呈现典型的膝痹病理改变。表明采用石膏铁丝固定法能成功建立兔膝痹模型,具有简单易行、固定牢固、无创伤等优点。
[Abstract]:Background: at present, the animal model of knee arthralgia is made by gypsum fixation method at home and abroad, but there are some problems such as poor selection of laboratory animals, improper selection of gypsum, instability of simple plaster fixation, etc. Objective: to establish rabbit knee arthralgia model by plaster wire fixation. Methods: twenty New Zealand white rabbits were randomly divided into two groups. In the model group, the right knee arthralgia model was established by winding the iron wire in the outer layer on the basis of the resin gypsum flexion position fixation, while the control group was the normal control group, and the control group was 8 weeks after fixation. Results and conclusion: in the control group, the knee joint was translucent, smooth, glossy, without cartilage defect, with good cartilage elasticity and hardness, and normal arrangement of chondrocytes. In the model group, the articular cartilage became thin, the elasticity was poor, the articular cartilage became degenerative, the articular surface became rough, there were some defects, and there was only a small amount of joint fluid or no joint fluid in the model group. Chondrocytes atrophy or disappearance, cartilage degeneration, necrosis, bone sclerosis, bone trabecular hyperplasia, disorder of chondrocytes, vitreous degeneration, synovial villi hypertrophy, cell clustering, and a large number of lymphocytes can be seen. The infiltration of plasma cells and neutrophils showed typical pathological changes of knee arthralgia, which indicated that the rabbit knee arthralgia model could be successfully established by using plaster wire fixation method, which had the advantages of simplicity, firm fixation and no trauma.
【作者单位】：新疆医科大学中医学院;新疆维吾尔自治区中医医院;
【基金】：新疆维吾尔自治区自然科学基金项目(2014211C007)~~
【分类号】：R-332;R684.3

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