内吗啡肽1抑制人外周血来源树突状细胞成熟和功能

发布时间：2018-03-18 03:00

本文选题：内吗啡肽(EM-)　切入点：树突状细胞　出处：《细胞与分子免疫学杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的研究加入脂多糖(LPS)刺激后内吗啡肽1(EM-1)对外周血来源树突状细胞(PBDC)的表型、细胞因子分泌和刺激T细胞增殖等方面的影响,探讨EM-1调节DC功能的可能作用途径及机制。方法用重组人粒细胞巨噬细胞集落刺激因子(rh GM-CSF)和重组人白细胞介素4(rh IL-4)联合刺激经培养和离心处理后的PBDC,分组加入LPS或EM-1刺激,观察细胞生长形态变化;流式细胞术检测PBDC表面CD80、CD86、CD83、HLA-DR、CC趋化因子受体7(CCR7)的水平;ELISA检测PBDC分泌的IL-10、IL-12、γ干扰素(IFN-γ)的水平;羟基荧光素二乙酸盐琥珀酰亚胺脂(CFSE)染色检测EM-1诱导后PBDC对T细胞增殖的影响。结果 EM-1使LPS刺激诱导后PBDC表面CD80、CD86、CD83、HLA-DR、CCR7表达下调;EM-1抑制LPS诱导成熟的PBDC释放炎性因子IL-12和IL-10,抑制PBDC对T淋巴细胞的增殖反应能力,减少PBDC和T淋巴细胞共培养上清液中IL-12和IFN-γ的分泌。结论 EM-1可抑制PBDC的成熟和功能。
[Abstract]:Objective to study the effects of LPS-stimulated morphine peptide 1 (EM-1) on the phenotype, cytokine secretion and T cell proliferation of peripheral blood derived dendritic cells (PBDCs). Methods EM-1 was combined with recombinant human granulocyte macrophage colony stimulating factor (rh GM-CSF) and recombinant human interleukin-4 (rhIL-4) to stimulate the cultured and centrifuged PBDCs. The level of HLA-DRCC chemokine receptor 7 (CCR7) on the surface of PBDC was detected by flow cytometry and the levels of IL-10 IL-12 and IFN- 纬 secreted by PBDC were detected by Elisa. Hydroxyfluorescein diacetate succinimide (EM-1) staining was used to detect the effect of PBDC on T cell proliferation induced by EM-1. Results the expression of CD80 CD86, CD86, CD83, HLA-DRCCR7 on PBDC surface stimulated by LPS was down-regulated by EM-1 to inhibit LPS induced PBDC release inflammatory factor. IL-12 and IL-10 inhibit the proliferation response of PBDC to T lymphocytes. The secretion of IL-12 and IFN- 纬 in supernatant of PBDC and T lymphocyte coculture was decreased. Conclusion EM-1 can inhibit the maturation and function of PBDC.
【作者单位】：蚌埠医学院生理学教研室;安徽省感染与免疫重点实验室;
【基金】：国家自然科学基金(81472656) 安徽省自然科学基金(1508085MH170) 安徽省教育厅自然科学研究项目(KJ2015B036by) 安徽省高校自然科学研究重点项目(KJ2016A481)
【分类号】：R392.12

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