金属蛋白质组学研究锌离子匮乏对肺炎链球菌的影响

发布时间：2018-03-18 19:28

本文选题：肺炎链球菌　切入点：金属蛋白　出处：《微生物学通报》2017年09期 　论文类型：期刊论文

【摘要】：【目的】研究锌离子缺乏对肺炎链球菌的影响,找到其适应性生长机制。【方法】以肺炎链球菌为模型,利用加锌和不加锌的培养基对细菌进行培养,收集细胞蛋白,采用双向凝胶电泳,结合金属亲和层析和质谱技术鉴定差异表达蛋白,进而通过生物信息学分析蛋白质相互关系,从中找到细菌适应锌离子匮乏条件的关键代谢通路和蛋白。【结果】测定了在限制培养条件下肺炎链球菌的最适生长浓度,建立了锌离子调控蛋白双向凝胶电泳图谱,鉴定到了96个差异表达蛋白斑点,共67个差异蛋白,其中32个表达下调,35个表达上调,锌离子调控蛋白的作用可能主要体现在糖代谢、核酸代谢、氧化还原作用、辅助蛋白质翻译、合成及折叠等方面。建立了锌结合蛋白的差异表达图谱,鉴定到了10个差异表达蛋白斑点,共7个差异蛋白,其中1个表达下调,6个表达上调。锌离子结合蛋白的作用可能主要体现在应对压力、蛋白质折叠和转运、氨基酸代谢等方面。【结论】肺炎链球菌主要通过调控碳水化合物代谢和核酸代谢等多个代谢通路来应对宿主锌金属离子匮乏的环境,从而使自身能够存活并对宿主形成感染。本研究为揭示细菌在宿主环境,特别是金属离子匮乏条件下的适应性生长机制提供理论基础。
[Abstract]:[objective] to study the effect of zinc deficiency on Streptococcus pneumoniae and to find out its adaptive growth mechanism. [methods] taking Streptococcus pneumoniae as a model, the bacteria were cultured with and without zinc, and the cell proteins were collected. Two-dimensional gel electrophoresis, metal affinity chromatography and mass spectrometry were used to identify differentially expressed proteins, and then bioinformatics was used to analyze the relationship between proteins. The key metabolic pathways and proteins of bacteria adapted to the condition of zinc deficiency were found. [results] the optimum growth concentration of Streptococcus pneumoniae under limited culture conditions was determined, and a two-dimensional gel electrophoresis map of zinc ion regulatory protein was established. 96 differentially expressed protein spots were identified, of which 32 were down-regulated and 35 up-regulated. The role of zinc ion regulatory proteins may be mainly reflected in glucose metabolism, nucleic acid metabolism, redox. The differential expression map of zinc binding protein was established and 10 differentially expressed protein spots were identified. One of them was down-regulated and six of them were up-regulated. Zinc ion-binding proteins may play an important role in coping with stress, protein folding and transport. Amino acid metabolism. [conclusion] Streptococcus pneumoniae mainly regulate carbohydrate metabolism and nucleic acid metabolism and other metabolic pathways to deal with the host zinc metal ion deficiency environment. This study provides a theoretical basis for revealing the adaptive growth mechanism of bacteria in host environment, especially in the absence of metal ions.
【作者单位】：暨南大学生命与健康工程研究院;
【基金】：广东省自然科学基金项目(No.2015A030313334) 广州市科技计划项目(No.201607010228)~~
【分类号】：R378.12

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