比格犬肝门静脉置管并体外留置模型的建立

发布时间：2018-03-20 08:32

本文选题：比格犬　切入点：吸入麻醉　出处：《军事医学》2017年04期 　论文类型：期刊论文

【摘要】：目的建立比格犬肝门静脉置管并体外留置模型。方法将6只比格犬行气管插管吸入麻醉,取右腹直肌切口,定位肠系膜上静脉及肝门静脉主干,游离、切断肠系膜上静脉左侧属支,将中心静脉导管(PICC)插入并置于肝门静脉主干内,结扎固定。导管末端经右侧腹壁皮下引至颈背部穿出并固定。结果实验组6只比格犬麻醉效果良好,手术全部成功,平均手术时间约1 h,平均出血量约15 ml,术后5~7 d手术切口愈合。结论比格犬肝门静脉置管并体外留置模型的成功建立,可提高肝脏的靶向性用药药效、减少用药剂量、降低治疗或实验成本并减少药物不良反应;通过置管可直接采集肝门静脉血测得口服药物或毒物在首过消除之前的血药浓度,间接计算胃肠吸收率,为开展相关实验研究提供技术支持。
[Abstract]:Objective to establish a model of intrahepatic portal vein catheterization and indwelling in vitro. Methods six Beagle dogs were anesthetized by tracheal intubation, the incision of right rectus abdominis muscle was taken, the superior mesenteric vein and the main portal vein of liver were located and dissociated. The left generic branch of superior mesenteric vein was cut off, and the central venous catheter PICCwas inserted into the main portal vein of the liver. The end of the catheter was subcutaneously led to the neck and back through the right abdominal wall and fixed. Results the anesthesia effect of 6 Beagle dogs in the experimental group was good and the operation was successful. The average operation time was about 1 hour, the average bleeding volume was about 15 ml, and the incision healed at 5 and 7 days after operation. Conclusion the successful establishment of the model of liver portal vein tube and indwelling in vitro in Beagle canine can improve the targeting effect of the liver and reduce the dosage of the drug. Reducing the cost of treatment or experiment and reducing adverse drug reactions; directly collecting hepatic portal vein blood to determine the blood concentration of oral drugs or poisons before first pass elimination, indirectly calculating gastrointestinal absorption rate, To provide technical support for related experimental research.
【作者单位】：军事医学科学院附属医院;航天中心医院急诊科;军事医学科学院科技部;军事医学科学院实验动物中心;
【基金】：国家科技支撑计划项目(2012BAI38B04)
【分类号】：R-332;R614

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