二乙基亚硝胺在小鼠诱发性肝癌模型中的应用

发布时间：2018-03-20 11:26

本文选题：二乙基亚硝胺　切入点：诱发性肝癌　出处：《中国比较医学杂志》2016年01期 　论文类型：期刊论文

【摘要】：DEN二乙基亚硝胺(diethylnitrosamine,DEN)对人体和动物都存在剧毒性,即使是小剂量注射或口服给药也会造成严重的肝损伤。由于对啮齿类动物诱导肝脏病变的稳定性,DEN常用于观察啮齿类动物肝癌形成全过程中形态学的改变以检测其癌前病变。在应用DEN后小鼠产生的肝损伤会引起肝硬化和肿瘤,使其成为分析人类肝细胞癌的发病机制和过程的理想动物模型。然而,一些研究表明,二乙基亚硝胺的致癌作用可能与动物不同遗传背景,性别,年龄等其他因素相关。因此,本文通过对相关文献进行综述,以期建立稳定,成功率高,死亡率低的DEN模型,并对不同目的的实验提供参考。
[Abstract]:DEN diethylnitrosamine (DEN) is highly toxic to human beings and animals. Even low dose injection or oral administration can cause severe liver damage. Den is often used to observe morphological changes during the formation of liver cancer in rodents because of the stability of liver lesions induced by rodents. Precancerous lesions. Liver damage in mice after DEN can lead to cirrhosis and tumors. It is an ideal animal model for analyzing the pathogenesis and process of human hepatocellular carcinoma. However, some studies have shown that the carcinogenic effect of diethylnitrosamine may be related to other factors, such as animal genetic background, sex, age and so on. In order to establish a stable DEN model with a high success rate and a low mortality rate, this paper reviews the relevant literatures, and provides a reference for experiments of different purposes.
【作者单位】：第二军医大学中医系;
【基金】：国家自然科学基金项目(81503632)
【分类号】：R735.7;R-332

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