ABCA6缺失对生酮饮食喂养的小鼠代谢的影响

发布时间：2018-03-20 21:16

本文选题：ABCA　切入点：生酮饮食　出处：《南京医科大学学报(自然科学版)》2017年07期 　论文类型：期刊论文

【摘要】：目的:检测ATP转运蛋白6(ATP binding cassette transporter A6,ABCA6)基因缺失对生酮饮食喂养的小鼠代谢的影响。方法:给予小鼠生酮饮食(ketogenic diet,KD)或普通饮食(normal diet,ND)2周,通过Western blot检测肝脏ABCA6的表达水平;给予ABCA6敲除(KO)小鼠或对照(WT)小鼠KD或ND 2周,检测小鼠的体重、血糖、血清甘油三酯(TG)、胆固醇(TCH)、游离脂肪酸(FFA)、酮体(β-HB)和转氨酶(AST、ALT)等指标,测定肝脏TG和TCH含量,并通过real-time PCR检测代谢相关基因的表达水平。结果:生酮饮食诱导小鼠肝脏ABCA6的表达;在普通饮食条件下,ABCA6 KO小鼠与对照小鼠在体重、血糖及血清TG、TCH、FFA、β-HB等代谢相关指标、肝脂质含量和代谢相关基因表达水平等方面无明显差别;在生酮饮食条件下,和WT小鼠相比,ABCA6 KO小鼠的血糖、FFA和TCH降低,肝脏TG蓄积更显著,肝脏FAS、DGAT2、PCK1和HMGCS2 mRNA显著增加。结论:ABCA6缺失引起小鼠对生酮饮食的代谢障碍,ABCA6可能参与肝脏的脂质代谢过程。
[Abstract]:Objective: to investigate the effect of the deletion of ATP transporter binding cassette transporter A6 on the metabolism of mice fed with ketone. Methods: the expression of ABCA6 in liver was detected by Western blot for 2 weeks in mice fed with ketogenic diettidine (KD) or normal diettidine (NDN). ABCA6 knockout mice or control mice were given KD or ND for 2 weeks. The body weight, blood glucose, serum triglyceride triglyceride (TG), cholesterol triglyceride (TG), free fatty acid (FFA), ketone body (尾 -HB) and transaminase (AST) were measured. Real-time PCR was used to detect the expression level of metabolism-related genes. Results: ketone diet induced the expression of ABCA6 in liver of mice, and the relative indexes of metabolism such as body weight, blood glucose, serum TGG TCHF FFAA, 尾 -HB and so on in normal diet were detected in ABCA6KO mice and control mice. There was no significant difference in the content of liver lipid and the expression level of metabolism-related genes in the liver, and the levels of FFA and TCH in the liver were lower than those in the WT mice, and the accumulation of TG in the liver was more significant than that in the WT mice. Liver FASA DGAT2 PCK1 and HMGCS2 mRNA were significantly increased. Conclusion ABCA6 may be involved in the lipid metabolism of lipids in mice due to the metabolic disorder of ketone diet induced by the absence of 1: ABCA6.
【作者单位】：南京医科大学病理生理学系;
【基金】：国家自然科学基金(81270944)
【分类号】：R33

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