非酒精性脂肪性肝病痰瘀互结证大鼠模型的构建及评价

发布时间：2018-03-21 03:15

本文选题：非酒精性脂肪性肝病　切入点：痰瘀互结证　出处：《中医杂志》2017年22期 　论文类型：期刊论文

【摘要】：目的探讨非酒精性脂肪性肝病(NAFLD)痰瘀互结证大鼠模型的构建。方法将20只SD大鼠随机分成对照组、模型组、经方组、协定方组,每组5只。除对照组外其余各组采用伤湿法加外界慢性轻度刺激、高脂饮食多因素复合刺激的方法建立NAFLD痰瘀互结证大鼠模型,连续10周。经方组和协定方治组于造模8周后给予相应中药1 ml/100 g灌胃,对照组和模型组均使用相同体积的生理盐水灌胃,每日1次,共2周。各组分别于造模1、10周进行舌象评分。造模10周进行肝脏HE染色,观察肝细胞脂肪变性程度;检测血脂、肝脏酶学指标及血液流变学指标。结果与对照组比较,模型组动物舌象评分、肝脏酶学指标、血脂、血液流变学指标均升高(P0.05)。经方组和协定方组舌下络脉评分较同时间模型组降低,血脂及肝脏酶学指标水平亦降低(P0.05);协定方组胆固醇(TC)、甘油三酯(TG)、丙氨酸氨基转移酶(ALT)水平较经方组下降明显(P0.05)。结论采用伤湿法加外界慢性轻度刺激、高脂饮食多因素复合刺激的方法建立的大鼠模型病理符合NAFLD,同时具有痰瘀互结证中医证候,中药方剂干预后反证造模成功。
[Abstract]:Objective to study the establishment of a rat model of phlegm and blood stasis syndromes in non-alcoholic fatty liver disease (NAF LDD). Methods Twenty SD rats were randomly divided into control group, model group, meridian group and agreement group. There were 5 rats in each group. The model of phlegm and blood stasis syndromes of NAFLD was established by the method of dampness injury and chronic mild stimulation and multiple factors of high fat diet stimulation in each group except the control group. After 8 weeks of model making, the two groups were given 1 ml/100 g of the corresponding Chinese medicine. The control group and the model group were given the same volume of normal saline once a day, and the control group and the model group were given the same volume of normal saline once a day, and the control group and the model group were given the same volume of normal saline. The liver was stained with HE for 10 weeks to observe the degree of steatosis of hepatocytes, blood lipid, liver enzyme and hemorheology were detected. The results were compared with those in the control group. The scores of tongue image, liver enzyme, blood lipid and hemorheology in the model group were all higher than those in the control group (P 0.05). The score of sublingual collaterals in the two groups was lower than that in the model group. The levels of serum lipids and liver enzymes were also decreased in the two groups, and the levels of TC, TGN and alt were significantly lower in the conventionally treated group than in the Jingfang group. Conclusion the method of damp-dampness plus chronic mild stimulation was used. The rat model established by the method of high fat diet and multifactor compound stimulation accords with NAFLD.The rat model has the syndrome of phlegm and blood stasis and the syndrome of phlegm and blood stasis. After intervention of traditional Chinese medicine prescription, the model of reverse syndrome is established successfully.
【作者单位】：西南医科大学附属中医医院;
【基金】：四川省教育厅科研计划(15ZB0156,14ZA0144) 泸州市财政局、泸州市科学技术和知识产权局2016年市级科技计划[2016-R-70(23/24)] 泸州医学院-泸州医学院附属中医院联合专项(2013ZRQN044)
【分类号】：R259;R-332

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