益肾通络方对肾纤维化模型大鼠肾组织转化生长因子-β1、Smad7表达的影响

发布时间：2018-03-21 14:52

本文选题：肾纤维化　切入点：益肾通络方　出处：《中医杂志》2016年11期 　论文类型：期刊论文

【摘要】：目的探讨益肾通络方治疗肾纤维化的可能作用机制。方法采用阳离子牛血清白蛋白皮下和尾静脉注射建立肾纤维化大鼠模型,将造模成功的50只大鼠随机分为模型组、对照组和益肾通络方低、中、高剂量组,每组各10只,并设10只同批正常大鼠为正常组。益肾通络方低、中、高剂量组分别予益肾通络方生药4.58、9.16、18.32 g/(kg·d)灌胃,对照组以肾炎四味片1.58 g/(kg·d)灌胃,模型组和正常组以等容量(1 ml/100 g)的蒸馏水灌胃,每天灌胃1次,连续4周。采用免疫组织化学方法结合图像分析系统测定大鼠肾组织转化生长因子-β1(TGF-β1)、Smad7的平均灰度值、平均光密度值、阳性细胞数、阳性率,并进行相关性分析。结果光镜下模型组肾组织TGF-β1表达明显增多,Smad7表达不明显。与模型组比较,益肾通络方各剂量组和对照组肾组织TGF-β1平均灰度值、阳性率、阳性细胞数明显减少,Smad7平均灰度值、阳性率、阳性细胞数明显提高,并且以益肾通络方中、高剂量作用更明显(P0.05或P0.01)。相关分析显示,TGF-β1与Smad7呈线性负相关(P0.01)。结论益肾通络方治疗肾纤维化可能机制是调节TGF-β1与Smad7的相互作用关系,促进Smad7表达,抑制TGF-β1表达。
[Abstract]:Objective to explore the possible mechanism of Yishen Tongluo recipe in the treatment of renal fibrosis. Methods the model of renal fibrosis was established by subcutaneous and caudal vein injection of cationic bovine serum albumin (BSA), and 50 rats were randomly divided into model group. The control group and Yishen Tongluo prescription group (10 rats in each group) were given intragastric administration of Yishen Tongluo prescription (Yishen Tongluo prescription 4.58N9.169.160.32 g / kg 路d), and 10 normal rats in the same batch were used as the normal group, respectively, in the control group and the Yishen Tongluo prescription group (n = 10 in each group), and 10 normal rats in the same batch were given intragastric administration of Yishen Tongluo recipe respectively. In the control group, 1.58 g / kg / d of Shenyan Siwei tablet was administered to the stomach, and the model group and the normal group were fed with distilled water of equal volume (1 ml/100 / g), once a day. For 4 weeks, the mean gray value, average optical density, number of positive cells and positive rate of TGF- 尾 _ 1 TGF- 尾 _ 1 Smad7 in rat renal tissue were determined by immunohistochemical method and image analysis system. Results the expression of TGF- 尾 1 in renal tissue of the model group was significantly increased and the expression of Smad7 was not obvious under light microscope. Compared with the model group, the average gray value of TGF- 尾 1 and the positive rate of TGF- 尾 1 in each dose group and control group of Yishen Tongluo recipe were higher than those in the model group. The number of positive cells significantly decreased the average gray value of Smad7, the positive rate and the number of positive cells increased significantly, and in Yishen Tongluo recipe, The correlation analysis showed that there was a linear negative correlation between TGF- 尾 1 and Smad7. Conclusion the possible mechanism of Yishen Tongluo recipe in treatment of renal fibrosis is to regulate the interaction between TGF- 尾 1 and Smad7, promote the expression of Smad7 and inhibit the expression of TGF- 尾 1.
【作者单位】：湖南师范大学医学院;湖南省肿瘤医院;中国人民解放军总医院;
【基金】：湖南省教育厅科学研究项目(14A094) 湖南省自然科学基金项目(14JJ4033) 湖南省中医药科研计划项目(201407)
【分类号】：R285.5;R-332

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