兔骨髓间充质干细胞向胰岛细胞分化的研究

发布时间：2018-03-21 18:17

本文选题：兔　切入点：BMSCs　出处：《畜牧兽医学报》2013年04期 　论文类型：期刊论文

【摘要】：旨在探讨体外诱导兔骨髓间充质干细胞向胰岛细胞分化的可行性,本研究采用二甲基亚砜联合高浓度葡萄糖对分离纯化的第3代兔BMSCs进行体外诱导,观察诱导前后细胞形态学变化,用双硫腙染色法鉴定胰岛细胞,用免疫细胞化学法检测胰岛素的表达,用ELISA法检测分泌到培养基中的胰岛素含量。结果,试验组在诱导7d出现细胞团样集落,10d获得排列紧密的细胞团,而对照组始终未见细胞团;经双硫腙染色试验组、免疫细胞化学染色试验组均显示阳性,对照组为阴性,ELISA检测试验组7和10d的胰岛素含量分别为(27.82±0.43)和(27.89±0.25)mU.L-1,与对照组的(23.39±0.20)和(25.71±0.27)mU.L-1比较,差异极显著(P0.01)。结果表明,二甲基亚砜联合高糖能够在较短的周期内诱导出胰岛细胞。
[Abstract]:To explore the feasibility of inducing rabbit bone marrow mesenchymal stem cells to differentiate into islet cells in vitro, dimethyl sulfoxide (DMSO) combined with high concentration glucose was used to induce the third generation rabbit BMSCs in vitro. The changes of cell morphology were observed before and after induction. Islet cells were identified by dithizone staining, insulin expression was detected by immunocytochemistry and insulin content was detected by ELISA method. After 7 days of induction, the cells in the experimental group were closely arranged, but the cells in the control group were not seen at all, and the immunocytochemical staining group were all positive after dithizone staining. The insulin contents in the control group were 27.82 卤0.43) and 27.89 卤0.25 mU.L-1, respectively, which were significantly higher than those in the control group (23.39 卤0.20) and the control group (25.71 卤0.27)mU.L-1). The results showed that dimethyl sulfoxide combined with high glucose could induce islet cells in a short period of time.
【作者单位】：河南农业大学牧医工程学院;开封市动物疫病预防控制中心;河南省动物卫生监督所;
【基金】：河南省基础与前沿技术研究计划(092300410081) 河南农业大学人才引进基金
【分类号】：S829.1

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