升降散治疗血管性痴呆模型大鼠的疗效及作用机制

发布时间：2018-03-22 13:49

本文选题：升降散　切入点：血管性痴呆　出处：《中国老年学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的探讨升降散治疗血管性痴呆模型大鼠的临床效果及作用机制。方法 SD大鼠随机分为正常组、假手术组、模型组和升降散组,假手术组大鼠仅切开颈前,模型组和升降散组大鼠行2-AO法永久性结扎双侧颈总动脉得到血管性痴呆模型大鼠。应用Morris水迷宫检测各组大鼠学习记忆能力,免疫荧光组织化学染色检测血管内皮生长因子(VEGF)阳性细胞数和计数微血管密度(MVD),细胞凋亡检测采用TUNEL法,采用SABC免疫组织化学法检测Bcl-2,Bax蛋白阳性表达。结果给药后,升降散组和模型组大鼠的学习次数高于假手术组、正常组,正确次数均显著低于假手术组、正常组(P0.05),升降散组大鼠学习次数低于模型组,正确次数显著高于模型组(P0.01);正常组和假手术组大鼠仅有少量VEGF阳性细胞,升降散组阳性细胞数量最高,显著高于其他三组(P0.01);升降散组海马CA1区凋亡细胞数显著低于模型组而显著高于正常组和假手术组(P0.01);海马CA1区Bcl-2和Bax阳性细胞数模型组大鼠高于正常组和假手术组(P0.01),升降散组大鼠海马CA1区Bax阳性细胞数明显低于模型组而Bcl-2阳性细胞数明显高于模型组,差异均有统计学意义(P0.01)。结论升降散可促进脑部血管再生,可通过下调Bax蛋白和上调Bcl-2蛋白表达以抑制脑细胞的凋亡,显著改善痴呆大鼠的学习、记忆能力。
[Abstract]:Objective to investigate the clinical effect and mechanism of the treatment of vascular dementia model rats. Methods SD rats were randomly divided into normal group, sham-operation group, model group and Sheng-shang powder group. The model rats of vascular dementia were obtained by permanent ligation of bilateral common carotid arteries by 2-AO in the model group and the Shengsheng powder group. The learning and memory abilities of the rats in each group were measured by Morris water maze. The number and microvessel density of VEGF positive cells were detected by immunofluorescence histochemical staining. Apoptosis was detected by TUNEL method, and Bcl-2P Bax protein expression was detected by SABC immunohistochemical method. The learning times of the rats in the Shengsheng powder group and the model group were higher than those in the sham operation group, and the correct times in the normal group were significantly lower than those in the sham operation group, and the learning times in the normal group were lower than those in the model group. The correct times were significantly higher than that in the model group (P 0.01), the number of VEGF positive cells in the normal group and the sham operation group was the highest, and the number of the positive cells in the Shengsheng San group was the highest. The number of apoptotic cells in hippocampal CA1 in Shengshangsan group was significantly lower than that in model group but higher than that in normal group and sham operation group, and the number of Bcl-2 and Bax positive cells in hippocampal CA1 region in model group was higher than that in normal group and sham operation group. The number of Bax positive cells in hippocampal CA1 region of rats in Shengsheng San group was significantly lower than that in model group, and the number of Bcl-2 positive cells in model group was significantly higher than that in model group. Conclusion Shengshangsan can promote cerebral vascular regeneration and inhibit the apoptosis of brain cells by down-regulating Bax protein and up-regulating the expression of Bcl-2 protein, and improving the ability of learning and memory in dementia rats.
【作者单位】：徐州市中医院脑病科;南京中医药大学基础医学院;
【分类号】：R285.5;R-332

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