制备和检测胚胎显微操作用针的实用方法体系

发布时间：2018-03-22 22:08

本文选题：显微操作　切入点：持卵针　出处：《西安交通大学学报(医学版)》2017年06期 　论文类型：期刊论文

【摘要】：目的介绍一种优化的制备及检测胚胎显微操作用针的实用方法体系。方法移卵管制作是用普通毛细管在酒精灯上拉制,小砂轮断针,针口用酒精灯火焰稍处理后备用;持卵针是将外径1.0mm的毛胚管在拉针仪上拉制,小砂轮断针,烧针仪上将开口处理成平滑内陷的马蹄形;注射针是将带内芯的外径1.0mm的特制毛胚管在拉针仪上拉制而成。注射针尖端充满注射溶液后,将注射针和持卵针连接到显微操作系统的操作臂上,先置于胚胎培养液内,用针尖轻轻碰触持卵针以破坏开口。之后转移到矿物油里,加压试注射,根据注射针开口液滴流出速度判断针尖是否通畅,流速是否合适。进一步用受精卵试注射进行验证。结果通过本方法体系制备的移卵管、持卵针和注射针均达到胚胎显微操作要求,解决了注射针口液体流速难以判断的问题。结论该胚胎显微操作用针的制备与检测方法体系使胚胎显微注射的前期工作得以标准化,提高了后续胚胎显微注射的效率。
[Abstract]:Objective to introduce an optimized method system for the preparation and detection of needles for micromanipulation of embryos. Methods the oval-moving tube was made with ordinary capillary tube drawn on the wine lamp, the needle was broken by small grinding wheel, and the needle mouth was slightly treated with the flame of wine lamp. The egg holding needle is to pull the outer diameter 1.0mm 's hairy embryo tube on the needle pulling instrument, the small grinding wheel breaks the needle, and the needle burner treats the opening into a smooth invagination horseshoe shape. The injection needle is made by pulling a special hairy embryo tube with the inner core of the outer diameter 1.0mm on the needle puller. After the injection needle tip is filled with injection solution, the injection needle and the egg holding needle are connected to the operating arm of the microoperating system and placed in the embryo culture medium. Touch the egg needle lightly with the needle to destroy the opening. Then transfer to the mineral oil, test the injection under pressure, and judge whether the needle tip is smooth according to the velocity of the liquid drop flowing out of the opening of the needle. The velocity of flow was appropriate. Further verification was made by fertilized egg test injection. Results the egg transfer tube, holding needle and injection needle prepared by this method met the requirements of embryo micromanipulation. Conclusion the preparation and detection system of needle for embryo microsurgery can standardize the early stage of embryo microinjection and improve the efficiency of subsequent embryo microinjection.
【作者单位】：陕西省人民医院新生儿科;西安交通大学医学部实验动物中心;
【基金】：国家自然科学基金资助项目(No.81370379) 陕西省自然科学基金资助项目(2016JM8061)~~
【分类号】：R-332

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