一种基于环介导等温扩增技术原理的组织胞浆菌分子诊断技术

发布时间：2018-03-24 09:03

  本文选题：组织胞浆菌　切入点：环介导等温扩增技术　出处：《中国真菌学杂志》2016年02期

【摘要】：目的研发一种基于环介导等温扩增(1oop-mediated isothermal amplification,LAMP)技术原理的组织胞浆菌感染的分子诊断技术。方法以组织胞浆菌为研究对象,针对其种特异性的M抗原基因序列,设计并筛选数套基于LAMP技术的特异性引物,同时优化其反应条件,建立一种针对组织胞浆菌感染的快速分子鉴定技术。结果该LAMP技术在65℃、90min反应条件下,对组织胞浆菌基因组DNA的检测敏感度达到5.3pg/反应(即5.3×10-12 g,约160个基因组DNA拷贝),且与其相近种属真菌无交叉反应。结论我们研发的这种基于LAMP技术原理的组织胞浆菌分子诊断技术具有操作简便、特异性及敏感性高等优点,具有临床推广应用的潜力。
[Abstract]:Objective to develop a molecular diagnostic technique based on the principle of cycle-mediated isothermal amplification of isothermal amplificationlampp. Methods the specific M antigen gene sequence of histiocytoplasmic bacteria was studied. Several sets of specific primers based on LAMP technique were designed and screened. At the same time, the reaction conditions were optimized, and a rapid molecular identification technique for histoplasmic bacteria infection was established. Results the LAMP technique was used at 65 鈩，

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