维药异黑成熟颗粒对异常黑胆质型慢性阻塞性肺疾病大鼠模型炎性反应的影响

发布时间：2018-03-24 10:40

本文选题：慢性阻塞性肺疾病　切入点：异常黑胆质型　出处：《中华中医药杂志》2017年10期

【摘要】：目的:研究异黑成熟颗粒对异常黑胆质型慢性阻塞性肺疾病(COPD)大鼠模型炎性反应的影响。方法:用熏烟90d结合气道滴注弹性蛋白酶法建立COPD,在此基础上叠加干寒环境、干寒饮食和足底电刺激建立异常黑胆质型COPD(ASSCOPD)。ASSCOPD于建模后第90天随机分为异黑组和异黑成熟颗粒干预组(给药组),给药组予异黑成熟颗粒灌胃0.54g·200g-1·d-1,共7d。ELISA法检测模型血清和肺泡灌洗液(BALF)中白细胞介素(IL)-8、IL-10、TNF-α。数据用SPSS 17.0统计软件统计分析。结果:(1)血清中炎性因子:与对照组比较:异黑组IL-8含量显著升高(P0.05)、IL-10、TNF-α含量显著降低(P0.01,P0.05),给药组TNF-α含量显著降低(P0.05)。与模型组比较,异黑组和给药组TNF-α含量显著降低(P0.05,P0.01),异黑组IL-8含量显著升高(P0.05)。与异黑组比较,给药组IL-10含量显著升高(P0.05)。(2)BALF中炎性因子:与对照组比较,模型组TNF-α含量显著升高(P0.01),异黑组IL-8、TNF-α含量显著升高(P0.05,P0.01),给药组IL-8含量显著升高(P0.05)。与模型组比较,给药组TNF-α含量显著降低(P0.05)。结论:异黑成熟颗粒可改善异常黑胆质型COPD大鼠模型的免疫功能,减轻模型炎性反应状态。
[Abstract]:Objective: to study the effect of Yi-mai mature granule on inflammatory response of rats with abnormal black gallbladder type chronic obstructive pulmonary disease (COPDD). Methods: COPDs were established by smoke smoke for 90 days combined with airway drip elastase method, on which the dry cold environment was superimposed. On the 90th day after the establishment of abnormal black gallbladder type COPD(ASSCOPD).ASSCOPD by dry cold diet and plantar electrical stimulation, the rats were randomly divided into two groups: the heteromonas group and the intervention group (the drug group was given the medicine group and the drug group was given 0.54 g 200g-1 路d -1). The model serum was detected by 7d.ELISA method. SPSS 17.0 statistical software was used to analyze the data. Results: compared with the control group, the content of IL-8 increased significantly in the isomerization group, and the content of IL-10 TNF- 伪 decreased significantly, and the content of TNF- 伪 in the drug group decreased significantly, and the content of TNF- 伪 in the control group was significantly decreased by using the statistical software of SPSS 17.0.Results: compared with the control group, the content of IL-8 in the isomelanoma group was significantly higher than that in the control group, and the content of TNF- 伪 in the administration group was significantly lower than that in the control group. Compared with the model group, the P0. 05% decreased significantly. The content of TNF- 伪 decreased significantly in the isomelanoma group and the administration group, and the IL-8 content in the isomelanoma group increased significantly compared with that in the isoblack group. Compared with the control group, the content of IL-10 in the administration group was significantly higher than that in the control group, and the content of IL-10 in the administration group was significantly higher than that in the control group, and compared with that in the control group, the content of TNF- 伪 was significantly increased in the treatment group. The content of TNF- 伪 in the model group was significantly higher than that in the control group (P 0.01), the content of IL-8 TNF- 伪 in the isomerization group was significantly higher than that in the control group, and the content of IL-8 in the administration group was significantly higher than that in the model group. Conclusion: the content of TNF- 伪 in the treatment group can improve the immune function and alleviate the inflammatory reaction of the model of COPD.
【作者单位】：新疆医科大学附属中医医院国家中医临床研究基地;新疆呼吸病研究重点实验室;
【基金】：新疆维吾尔自治区科技创新团队培养建设项目(No.2014751005)~~
【分类号】：R29;R-332

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