归芪聪志汤对血管性痴呆大鼠模型脑组织氧化应激损伤的影响

发布时间：2018-03-24 14:25

本文选题：血管性痴呆　切入点：氧化应激　出处：《中国老年学杂志》2017年18期

【摘要】：目的观察归芪聪志汤对血管性痴呆(VD)大鼠模型脑组织氧化应激损伤的作用。方法采用反复夹闭颈动脉加注射硝普钠降压再加单侧永久结扎颈总动脉法制备VD模型,从行为学、组织病理、氧化应激酶学三个方面观察归芪聪志汤对VD的影响。结果对比假手术组,模型组大鼠定位航行潜伏期明显延长,空间搜索穿越平台次数明显减少(P0.05),海马病理切片显示:细胞数量明显较少、细胞轮廓模糊皱缩、胞质深染、核不清晰,丙二醛(MDA)含量增加,超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性均明显降低;归芪聪志汤中剂量组中、高剂量组上述指标明显改变,尤以中剂量组效应最明显。对比模型组,中剂量归芪聪志汤组大鼠定位航行潜伏期明显变短,空间搜索穿越平台次数明显增多,海马病理切片显示细胞损伤减轻,MDA含量明显降低,SOD和GSH-Px活性明显升高;中剂量归芪聪志汤疗效类似于甚至优于西药脑复康。结论中剂量归芪聪志汤通过抗氧化应激作用保护VD大鼠脑组织,改善其病理损伤和记忆功能。
[Abstract]:Objective to observe the effect of Gui Qi Cong Zhi decoction on oxidative stress injury in brain tissue of rats with vascular dementia. Methods VD model was established by repeatedly clamping carotid artery, injecting sodium nitroprusside, lowering blood pressure and unilateral permanent ligation of common carotid artery. Histopathology and oxidative stress enzyme were used to observe the effect of Gui Qi Cong Zhi decoction on VD. Results compared with the sham operation group, the latency of positioning navigation in the model group was significantly longer than that in the sham operation group. The frequency of spatial search through the platform decreased significantly (P 0.05), and the hippocampal pathological sections showed that the number of cells was significantly less, the cell outline was blurred and shrinked, the cytoplasm was deep stained, the nucleus was not clear, and the content of malondialdehyde (MDA) MDAs was increased. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were significantly decreased in the middle dose group of Gui Qi Cong Zhi decoction, especially in the middle dose group, especially in the middle dose group. In the middle dose of Gui Qi Cong Zhi decoction group, the latency of localizing navigation was shortened, the times of space searching and traversing the platform increased obviously, and the pathological sections of hippocampus showed that the content of GSH-Px and the activity of GSH-Px were obviously increased after the cell injury was alleviated. Conclusion the middle dose of Gui Qi Cong Zhi decoction can protect the brain tissue of VD rats through antioxidant stress and improve its pathological damage and memory function.
【作者单位】：甘肃中医药大学基础医学院;
【基金】：甘肃省中药药理与毒理学重点实验室自主课题(ZDSYS-ZZKJ-2013[B]-004)
【分类号】：R285.5;R-332

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