基于气机升降理论观察旋覆代赭汤及其拆方对反流性食管炎模型大鼠食管线粒体ANT转运活性的影响
本文选题：反流性食管炎 切入点：旋覆代赭汤 出处：《辽宁中医杂志》2016年08期
【摘要】：目的:观察旋覆代赭汤及其拆方对反流性食管炎(RE)模型大鼠线粒体腺苷酸转运体(ANT)转运活性的影响。方法:将60只雄性Wistar大鼠,随机分成6组,即正常组、模型组、西药组,旋覆代赭汤全方组及拆方组(包括苦降组、甘升组),每组10只大鼠。对除正常组外的造模组大鼠采用"4.2 mm幽门夹+胃底2/3结扎术"制备RE动物模型。从术后第7天开始,正常对照组、模型对照组给予生理盐水灌胃,旋覆代赭汤全方组、拆方各组及西药组分别给予相应药液灌胃,干预14 d后,第22天处死全部大鼠后,取材,在光学显微镜下观察RE大鼠病理分级,各组随机取6份食管标本匀浆制成线粒体悬浮,液通过H3-ADP掺入法测量检测食管线粒体ANT转运活性。结果:各组大鼠食管黏膜病理形态学积分比较:模型组大鼠食管黏膜病理积分比正常组显著升高,有显著性差异(P0.01);全方组、西药组、甘升组食管黏膜病理形态学积分比模型组明显降低,差异有统计学意义(P0.05),苦降组食管黏膜病理形态学积分较模型组稍有降低,但差异无统计学意义(P0.05);全方组及西药组食管黏膜病理形态学积分较甘升组稍降低,但差异无统计学意义(P0.05)。各组食管线粒体ANT转运活性比较:与正常组比较,模型组、西药组、全方组、甘升组大鼠食管线粒体ANT转运活性略提高,差异无统计学意义(P0.05),苦降组略降低,差异无统计学意义(P0.05);与模型组比较,西药组、全方组及甘升组大鼠食管线粒体ANT转运活性略提高略升高,但差异无统计学意义(P0.05);西药组、全方组、甘升组及苦降组组间比较均无统计学意义(P0.05)。结论:旋覆代赭汤通过调节脾胃气机改善RE模型大鼠食管黏膜光镜下表现,促进黏膜修复,改善炎症。在一定程度上提高RE大鼠线粒体ANT转运活性,辅助线粒体能量的顺利完成,亦可能是旋覆代赭汤治疗RE的作用机制之一。
[Abstract]:Objective: to observe the effect of Xuanhudaizhe decoction and its disassembly on mitochondrial adenylate transporter (ANT) transport activity in rat model of reflux esophagitis. Methods: sixty male Wistar rats were randomly divided into 6 groups: normal group, model group and western medicine group. Xuanhudezhe decoction group and disassembly group (including Kujiang group, Gansheng group, 10 rats in each group). The rats in the model group other than the normal group were treated with "4.2 mm pyloric clamp stomach fundus 2 / 3 ligation" to make RE animal model. From the 7th day after operation, RE animal model was established. The normal control group and the model control group were given normal saline intragastric perfusion, Xinfen daizhe decoction group, the disassembled prescription group and western medicine group respectively. After 14 days of intervention, all the rats were killed on the 22nd day, and the materials were obtained. The pathological grades of RE rats were observed under optical microscope. Six esophageal homogenates were randomly selected for mitochondrial suspension in each group. Results: compared with the normal group, the pathological score of esophageal mucosa in the model group was significantly higher than that in the normal group (P 0.01). In the western medicine group, the pathological morphology integral of the esophageal mucosa in the Gan-sheng group was significantly lower than that in the model group, and the difference was statistically significant (P 0.05). The pathological morphology integral of the esophageal mucosa in the Kujiang group was slightly lower than that in the model group. But the difference was not statistically significant (P 0.05), the score of pathomorphology of esophageal mucosa in the whole prescription group and the western medicine group was slightly lower than that in the Gan-sheng group, but the difference was not statistically significant (P 0.05). The comparison of the ANT transport activity of the esophageal mitochondria in each group: compared with the normal group, the model group, the western medicine group, the control group, the model group and the western medicine group, In the whole prescription group, the mitochondrial ANT transport activity of the rats in the Gan-sheng group increased slightly, but the difference was not statistically significant (P 0.05), but the difference was not significant in the bitter group (P 0.05), and compared with the model group, the western medicine group, the western medicine group, The activity of mitochondrial ANT transport increased slightly in the whole prescription group and the Gansheng group, but the difference was not statistically significant (P 0.05), while that in the western medicine group, the whole prescription group, the western medicine group, the whole prescription group, the western medicine group, the whole prescription group, There was no significant difference between Gan-sheng group and Ku-Jiang group (P 0.05). Conclusion: Xuanfudezhe decoction can improve the esophageal mucosal light microscope performance and promote mucosal repair by regulating spleen and stomach Qi. To improve inflammation, to some extent increase the activity of mitochondrial ANT transport in RE rats and assist the smooth completion of mitochondrial energy, which may also be one of the mechanisms of Xuanhudaizhe decoction in the treatment of RE.
【作者单位】： 天津市南开医院消化内科;天津中医药大学;
【基金】：国家自然科学基金项目(81173243)
【分类号】：R285.5;R-332
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