CXCR4基因转染对骨髓间充质干细胞体外生物学行为的影响

发布时间：2018-03-28 01:27

本文选题：骨髓间充质干细胞　切入点：趋化因子受体CXCR　出处：《中国药理学通报》2017年06期

【摘要】：目的观察对转染CXCR4基因的骨髓间充质干细胞体外生物学行为的影响。方法将培养的骨髓间充质干细胞分为3组,GFP组、CXCR4~+组、CXCR4~-组,转染趋化因子受体CXCR4,并用免疫荧光细胞化学法、流式细胞仪法及Transwell小室细胞趋化实验,体外研究了CXCR4高表达及低表达对MSCs增殖、分化与迁移能力的影响。结果 CXCR4高表达及低表达均不影响MSCs的增殖能力,对其向肺组织分化的能力也没有影响。与GFP-MSCs组相比,CXCR4~+-MSCs组迁移的细胞数量明显升高,而CXCR4~--MSCs组迁移细胞数量差异无显著性。结论 CXCR4高表达及低表达不改变MSCs的增殖、分化能力,然而CXCR4高表达明显增强MSCs向炎症病灶的迁移能力。说明CXCR4高表达的MSCs移植入体后将会更快速、更大量地到达病变区域参与组织修复,明显增强疗效。
[Abstract]:Objective to observe the effect of transfection of CXCR4 gene on the biological behavior of bone marrow mesenchymal stem cells (BMSCs) in vitro. Methods the cultured BMSCs were divided into three groups: CXCR4- group and CXCR4- group. The chemokine receptor CXCR4 was transfected into CXCR4- group, and the chemokine receptor CXCR4 was transfected into CXCR4- group by immunofluorescence cytochemistry. The effects of high and low expression of CXCR4 on the proliferation, differentiation and migration of MSCs were studied by flow cytometry and chemotaxis assay of Transwell chamber. Results the high expression and low expression of CXCR4 did not affect the proliferation of MSCs. Compared with GFP-MSCs group, the number of migration cells in CXCR4 ~ -MSCs group was significantly increased, but there was no significant difference in the number of migration cells in CXCR4~--MSCs group. Conclusion the high and low expression of CXCR4 does not change the proliferation and differentiation ability of MSCs. However, the high expression of CXCR4 significantly enhanced the ability of MSCs to migrate to inflammatory focus, which indicated that MSCs with high expression of CXCR4 would reach the lesion area more quickly and participate in tissue repair, and enhance the curative effect obviously.
【作者单位】：辽宁中医药大学药学院药理学教研室;大连医科大学;
【基金】：国家自然科学基金资助项目(No 81273923)
【分类号】：R329.2

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