共振光散射光谱法测定几种抗病毒和抗炎药物分子

发布时间：2018-03-28 04:32

  本文选题：共振光散射光谱法　切入点：厚朴酚　出处：《湘潭大学》2011年硕士论文

【摘要】：共振光散射技术的近些年在各分析领域应用较广泛,以方法灵敏度高、操作简便、仪器简单、分析速度快等许多优点引起科学研究者的注意。本文主要以抗炎和抗病毒药为研究对象,采用共振光散射技术并结合紫外吸收分光光度法和量子化学计算方法,研究了它们的光谱性质,影响因素及相关机理,从而建立了测定的新方法。 本文主要有五部分组成,具体内容如下: 第一章综述了共振光散射光谱(RLS)法的发展、原理、定量分析基础;以及厚朴酚与和厚朴酚、α-倒捻子素、柚皮素、山奈酚等分析的研究进展。 第二章研究了厚朴酚及和厚朴酚与阳离子染料结晶紫(CV)和阴离子表面活性剂十二烷基硫酸钠(SDS)形成的三元离子缔合物的RLS特性,结合紫外吸收分光光度法对三者之间的作用机理进行了初步探讨,并对一些影响因素进行了优化。在pH=3.5的HAc-NaAc缓冲溶液介质中,结晶紫对十二烷基硫酸钠与厚朴酚/和厚朴酚的共振光散射体系有协同增强作用。在333 nm共振波长处产生的RLS强度最强,在0.02～5.33μg/mL范围内呈较好的线性关系,检出限1.88×10~(-4)μg/mL,回收率为98.1%～103.0%,RSD为2.32% 第三章研究了茜素红(AR)-十六烷基三甲基溴化铵(CTMAB)-α-倒捻子素三元体系的紫外吸收光谱和RLS特性,以及影响因素和最佳实验条件。在pH=7.6的Na_2CO_3-NaHCO_3缓冲介质中,在331 nm△IRLS (体系的共振光强度变化)与α-倒捻子素在0.082～5.68μg/mL范围内呈线性关系,检出限为2.83 ng/mL,回收率97.8%～101.4%,RSD为1.7%。 第四章研究了铝(Ⅲ)-柚皮素-十二烷基苯磺酸钠(SDBS)三元体系的RLS特性,影响因素及最佳反应条件。实验结果表明,在pH=3.5的BR缓冲溶液中,在323 nm处△IRLS与柚皮素在0.027～6.80μg/mL范围内呈线性关系,检出限为1.9 ng/mL,回收率98.8%～101.0%,RSD为1.2%。 第五章研究了山奈酚的RLS特性,山奈酚在酸性条件下有很强的RLS值,而在碱性介质中共振光消失。在pH=5.5的BR缓冲介质中,并用量子化学计算方法对山奈酚分子内和分子间氢键进行了计算,初步探讨了山奈酚共振光散射光谱形成的机理。
[Abstract]:Resonance Light scattering (RLS) technology has been widely used in various analytical fields in recent years, with high sensitivity, simple operation and simple instrument. Many advantages, such as high speed of analysis, have attracted the attention of scientific researchers. In this paper, antiinflammatory and antiviral drugs are mainly studied, and resonance light scattering (RLS) technique, combined with ultraviolet absorption spectrophotometry and quantum chemical calculation, is used. Their spectral properties, influencing factors and related mechanisms were studied, and a new method for their determination was established. There are five parts in this paper, the specific contents are as follows:. In the first chapter, the development, principle and basis of quantitative analysis of resonance light scattering spectroscopy (RLSs) method are reviewed, as well as the research progress in the analysis of honokiol and honokiol, 伪 -inverted twist, naringin, kaempferol, etc. In chapter 2, the RLS properties of ternary ion-association complexes formed by honokiol and honokiol with cationic dye crystal violet and anionic surfactant sodium dodecyl sulfate were studied. Combined with ultraviolet absorption spectrophotometry, the mechanism of action among the three was preliminarily discussed, and some influencing factors were optimized. In the HAc-NaAc buffer solution of pH=3.5, Crystal violet has synergistic effect on the resonance light scattering system of sodium dodecyl sulfate and honokiol / honokiol. The intensity of RLS produced at 333nm is the strongest, and the linear relationship is good in the range of 0.02 渭 g/mL. The detection limit was 1.88 脳 10 ~ (-4) 渭 g 路mL ~ (-1) and the recovery was 98.1% and the RSD was 2.32%. In chapter 3, the UV absorption spectra and RLS characteristics of the ternary system of alizarin red AR-cetyltrimethylammonium bromide (CTMAB- 伪 -inverted twist), the influencing factors and the optimum experimental conditions are studied. In the Na_2CO_3-NaHCO_3 buffer medium of pH=7.6, There was a linear relationship between 伪 -inverted twist at 0.082 渭 g/mL and resonance light intensity change at 331nm. The detection limit was 2.83 ng / mL, and the recovery rate was 97.8ng / mL. The recovery was 1.7ng-1 路mL ~ (-1). In chapter 4, the RLS properties, influencing factors and optimum reaction conditions of Al (鈪，

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