半乳糖凝集素-1在子宫内膜异位症大鼠模型异位及在位内膜中的表达

发布时间：2018-03-28 22:30

  本文选题：半乳糖凝集素-　切入点：子宫内膜异位症　出处：《现代妇产科进展》2017年05期

【摘要】：目的:研究半乳糖凝集素-1(gal-1)在大鼠子宫内膜异位症(EMs)模型异位及在位组织中的表达。方法:选取12只性成熟雌性未孕Wistar大鼠,取一侧宫角子宫内膜,采用自体移植法建立EMs大鼠模型。术后28天再次开腹,将建模成功的大鼠作为自身对照,取其异位病灶与在位子宫内膜,苏木精-伊红(HE)染色鉴定组织学特征。免疫组织化学(IHC)法、实时荧光定量逆转录聚合酶链反应(qRT-PCR)及蛋白免疫印迹(Western blot)法检测异位病灶与在位内膜中gal-1表达。结果:肉眼观异位病灶呈椭圆形囊泡状,内含清亮或咖啡色液体,表面及周围有新生血管。组织学证实为异位内膜。造模成功率为83.33%。IHC显示,gal-1表达于异位及在位子宫内膜基质;QRT-PCR定量分析表明,异位内膜gal-1 mRNA表达量高于在位内膜(P0.05);Western blot结果示,异位内膜gal-1蛋白水平升高(P0.05)。结论:大鼠EMs异位内膜中gal-1表达高于在位内膜,为该靶点参与EMs的机制及治疗的研究打下基础。
[Abstract]:Objective: to study the expression of galactose agglutinin (galactose lectin) in ectopic and eutopic tissues of rats with endometriosis. The EMs rat model was established by autologous transplantation. After 28 days of operation, the successful model rats were used as self-control, and the ectopic focus and eutopic endometrium were taken out. Histopathological characteristics of hematoxylin-eosin (HEH) staining. The expression of gal-1 in ectopic lesions and eutopic endometrium was detected by real-time fluorescence quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and Western blot. There were neovascularization on and around the surface. Histologically confirmed ectopic endometrium. The successful rate of modeling was 83.33%.IHC and QRT-PCR quantitative analysis showed that the expression of gal-1 mRNA in ectopic and eutopic endometrial stroma was higher than that in eutopic endometrium (P 0.05), and the results of Western blot showed that the expression of gal-1 in ectopic endometrium was higher than that in eutopic endometrium. Conclusion: the expression of gal-1 in ectopic endometrium of EMs is higher than that in eutopic endometrium, which provides a basis for the study of the mechanism and therapy of EMs.
【作者单位】： 南方医科大学附属深圳市妇幼保健院;
【基金】：深圳市科创委资助项目(No:JCYJ20150402090412995) 深圳市卫计委项目(No:201501034)
【分类号】：R-332;R711.71
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