十一酸睾酮对大鼠肛提肌骨骼肌卫星细胞雄激素受体的影响

发布时间：2018-03-29 08:27

本文选题：骨骼肌卫星细胞　切入点：雄激素　出处：《山西医科大学》2011年硕士论文

【摘要】：目的探讨不同浓度雄激素对骨骼肌卫星细胞的作用和对雄激素受体的影响。方法原代培养骨骼肌卫星细胞,并鉴定细胞,通过应用5种不同浓度的雄激素(0.01×10-3ng/ul、0.04×10-3 ng/ul、0.16×10-3ng/ul、0.64×10-3ng/ul、1×10-3ng/ul)干预骨骼肌卫星细胞,测定骨骼肌卫星细胞的生长曲线、细胞的数量和目的细胞所表达雄激素受体含量的变化。结果在女性的正常生理范围内,随着雄激素浓度的升高,细胞数量增多,增殖加快,雄激素受体表达水平呈逐渐升高趋势,但不同浓度的雄激素对骨骼肌卫星细胞作用的差异不显著。结论雄激素作用于肛提肌中骨骼肌卫星细胞后,表现出雄激素促进骨骼肌卫星细胞增殖和AR的表达。在女性雄激素生理剂量范围内,随着雄激素浓度的增加,雄激素所表达的促进细胞增殖和AR表达的作用越强。
[Abstract]:Objective To study the effects of different concentrations of androgen on skeletal muscle satellite cells and androgen receptor .

Methods The skeletal muscle satellite cells were cultured in primary culture , and the growth curve of skeletal muscle satellite cells , the number of cells and the content of androgen receptor expressed in the target cells were determined by the intervention of five different concentrations of the androgen ( 0.01 脳 10 - 3 ng / ul , 0.04 脳 10 - 3 ng / ul , 0.16 脳 10 - 3 ng / ul , 0.64 脳 10 - 3 ng / ul , 1 脳 10 - 3 ng / ul ) .

Results In the normal physiological range of women , with the increase of androgen concentration , the number of cells increased , the proliferation was accelerated , the expression level of androgen receptor increased gradually , but the difference of the effect of androgen on skeletal muscle satellite cells was not significant .

Conclusion Androgen plays a role in promoting the proliferation of skeletal muscle satellite cells and the expression of AR after skeletal muscle satellite cells in levator ani muscle .

【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R329

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