口服牛Ⅱ型胶原蛋白诱发类风湿关节炎大鼠模型的建立及评价

发布时间：2018-03-30 00:09

本文选题：类风湿关节炎　切入点：食物抗原　出处：《江西师范大学学报(自然科学版)》2017年05期

【摘要】：按照动物建模方案分别给不同组大鼠灌胃一定剂量的阿司匹林,再给大鼠灌胃牛II型胶原蛋白诱导其产生类风湿关节炎;建模期间进行踝关节直径的测量及关节炎指数评分;然后ELISA实验检测大鼠血清中特异性抗体(IgE、IgG)和炎症因子(IL-6、TNF-α和IFN-γ);再进一步观察大鼠踝关节组织病理改变.结果表明:经过不同剂量阿司匹林(5、10 mg·kg-1)处理的大鼠,用牛II型胶原蛋白灌胃后,大鼠踝关节呈现不同程度的肿胀及关节炎症状;10 mg·kg-1处理的大鼠炎症更明显,其血清中特异性抗体(IgE、IgG)和炎性细胞因子IL-6、TNF-a也显著增加,而细胞因子IFN-r显著降低,同时踝关节组织也发生明显炎症病变.这表明10 mg·kg-1处理的大鼠要比5 mg·kg-1处理大鼠更易用于口服食物抗原牛II型胶原蛋白类风湿关节炎的建成.
[Abstract]:According to the animal modeling scheme, the rats in different groups were given a certain dose of aspirin, and then the rats were fed with bovine type II collagen to induce rheumatoid arthritis, the ankle diameter and arthritis index were measured and evaluated during the modeling period. Then ELISA assay was used to detect the specific antibody (IgEG) and the inflammatory factor (IL-6) TNF- 伪 and IFN- 纬 in the serum of rats, and the pathological changes of the ankle joint were observed further. The results showed that the rats treated with different doses of aspirin (5mg / kg ~ (-1)) were treated with TNF- 伪 and IFN- 纬, and the rats were treated with different doses of Aspirin (5mg / kg ~ (-1)). After the rats were perfused with bovine type II collagen, the swelling of ankle joint and the inflammation of rats treated with 10 mg kg-1 of arthritis symptoms were more obvious, and the specific antibody IgG) and the inflammatory cytokine IL-6 + TNF-a in serum were also significantly increased. However, the cytokine IFN-r was significantly decreased and the ankle tissue was also obviously inflamed, which indicated that the rats treated with 10 mg kg-1 were more suitable for the establishment of oral food antigen type II collagen rheumatoid arthritis than the rats treated with 5 mg kg-1.
【作者单位】：深圳市罗湖区人民医院风湿免疫科;深圳大学过敏反应与免疫学研究所;深圳市人民医院风湿免疫科;
【基金】：国家自然科学基金(81271950) 深圳市科技计划基础研究(JCYJ20130402151227168)资助项目
【分类号】：R-332;R593.22

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