盖塔病毒分子进化及其迁徙

发布时间：2018-03-30 09:44

本文选题：盖塔病毒　切入点：分子进化　出处：《中国人兽共患病学报》2017年04期

【摘要】：目的了解世界各地分离盖塔病毒分子进化特征及其时空迁徙。方法用Clustal X1.83、MegaAlign和Gene DOC软件对测定的核苷酸和推测的氨基酸序列进行比较分析,用MEGA 6.0软件绘制系统发生树,采用BEAST v 1.8.1软件包里的Bayesian Stochastic Search Variable Selection(BSSVS)程序进行盖塔病毒的空间动力学分析。结果盖塔病毒E2基因全长均为1 266nt,编码422aa,其核苷酸和氨基酸同源性分别为94.5%~100%和96.4%~100%。病毒分子进化分析发现,盖塔病毒不存在蚊虫,马匹和猪等宿主动物和媒介的种属和地域分布差异。生物信息学分析结果显示,盖塔病毒起源于马来西亚,此后依次传播至日本、中国、韩国以及蒙古国和俄罗斯等地。结论自1955年首次分离盖塔病毒至2014年间分离的盖塔病毒E2基因序列较为稳定,未发现病毒基因组之间存在种属及地域分布差异,并且该病毒已经从热带地区传播到欧亚大陆多个国家和地区,因此加强盖塔病毒及人畜动物感染的检测和监测至关重要。
[Abstract]:Objective to understand the molecular evolution characteristics and spatiotemporal migration of Gelta virus isolated from different parts of the world. Methods the nucleotides and deduced amino acid sequences were compared and analyzed with Clustal X1.83 MegaAlign and Gene DOC software, and the phylogenetic tree was plotted by MEGA 6.0 software. The spatial kinetic analysis of Gaeta virus was carried out by using the Bayesian Stochastic Search Variable selection (BSSVSs) program of BEAST v1.8.1. The results showed that the E2 gene of Gaeta virus was 1 266nt in length, encoding 422aa. The nucleotide and amino acid homology were 94.555% and 96.44%, respectively. Subevolution analysis found that, There are no differences in species and geographical distribution among host animals and vectors, such as mosquitoes, horses and pigs. Bioinformatics analysis shows that Gaeta virus originated in Malaysia and then spread to Japan, China, etc. Conclusion the E2 gene sequence of Gaeta virus isolated from 1955 to 2014 is relatively stable, and there are no species and regional distribution differences among the genomes of the virus. And the virus has been spread from the tropics to many countries and regions in Eurasia, so it is very important to strengthen the detection and surveillance of Gaeta virus and animal infection.
【作者单位】：山西医科大学;中国疾病预防控制中心病毒病预防控制所/传染病预防控制国家重点实验室;山东理工大学;
【基金】：国家自然科学基金重大项目(No.81290342)资助~~
【分类号】：R373

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