肝郁致精瘀实验动物模型研究

发布时间：2018-04-02 12:32

本文选题：肝郁　切入点：精瘀　出处：《成都中医药大学》2011年硕士论文

【摘要】：目的基于中医肝疏泄功能具有疏通畅达气机,调畅情志,协调冲任二脉,促进生殖之精排泄的理论,及前期研究组对败精(精瘀)理论、实验研究的基础上,本研究在使用慢性束缚应激法造成情志抑郁——肝气郁结的前提下,通过电动刺激棒建立雄性大鼠肝郁致精瘀的动物模型,以探讨情志——肝郁——精瘀的相关性,为探讨肝郁致精瘀的机理、肝郁精瘀的致病性、肝郁精瘀所致各种疾病的治疗提供依据。方法将75只雄性SD大鼠随机分为四组,即第一组为对照组30只,第二组为肝郁模型组15只,第三组为肝郁药物组15只,第四组为肝郁精瘀组15只。采用慢性束缚应激方法,将大鼠置于特制的束缚筒内4周建立肝郁模型的基础上,给第四组大鼠使用自制电动刺激棒刺激10日建立肝郁精瘀模型,并通过观察生殖性腺组织相关指标的改变以确立肝郁致精瘀模型的建立。结果研究发现：肝郁模型组、肝郁精瘀组与对照组大鼠相比较,行为多表现为倦怠少动、叫声尖细、反应迟缓、饮食减少、毛色晦暗发黄、大便颗粒松散甚至呈水样便等。对照组与肝郁模型组、肝郁药物组尾悬挂实验挣扎次数三组之间存在显著差异(p0.01)。大鼠血浆ACTH检测对照组与肝郁模型组、肝郁药物组三组之间存在显著差异(p0.05)。血浆CORT检测正常对照组与肝郁模型组、肝郁药物组三组之间存在着显著的差异(p0.05)。分别从药物治疗前后行为学表现、神经内分泌系统指标差异两个方面验证慢性束缚应激建立肝郁证大鼠模型的可靠性。对大鼠生殖腺组织的ACP检测(P0.05),及α-Glu检测(P0.05),肝郁精瘀组和正常对照组之间无明显差异。但造模过程中,使用电动刺激棒刺激后,大部分大鼠在静息状态下阴茎口内可见白色分泌物,符合中医白浊病证临床表现中滴白症状,在一定程度上表现出了精瘀的存在。结论本次研究采用慢性应激束缚筒制动及电动刺激棒刺激的方法,肝郁精瘀组大鼠表现出在刺激期间阴茎口有滴白现象,在一定程度上表现出了精瘀的存在,说明了肝郁与精瘀具有相关性,为进一步深入研究做了有意义的尝试。
[Abstract]:Purpose. Based on the theory that the function of traditional Chinese medicine (TCM) to soothe the liver and relieve the discharge of qi, regulate the emotion, coordinate the two veins, and promote the excretion of the essence of reproduction, and on the basis of the experimental study on the theory of sterility (blood stasis) in the early stage of the study group, In this study, on the premise that chronic restraint stress was used to cause depression of emotion and depression of liver-qi, the animal model of blood stasis induced by liver depression in male rats was established by electric stimulation rod, in order to explore the correlation between emotion, liver-depression and blood stasis. In order to study the mechanism of liver depression and blood stasis, the pathogenicity of liver stagnation and the treatment of various diseases caused by liver stagnation and essence stasis. Method. Seventy-five male SD rats were randomly divided into four groups: control group (n = 30), model group (n = 15), drug group (n = 15) and group IV (n = 15). On the basis of the model of liver-depression induced by Zhou Jianli in a special restraint tube, the rats in the fourth group were stimulated with self-made electric stimulator for 10 days to establish the model of liver depression and blood stasis. By observing the changes of related indexes of reproductive gonad tissue, the model of liver depression and essence blood stasis was established. Results. The results showed that compared with the control group, the rats in the liver depression model group and the liver depression essence stasis group were characterized by burnout, thin call, slow response, reduced diet, dark and yellowish coat color. There were significant differences in the number of experimental struggles between the control group and the liver depression model group and the liver depression drug group. There was a significant difference among the three groups (p 0.01). The plasma ACTH was detected in the control group and the liver depression model group. There was a significant difference among the three groups in liver depression drug group (P 0.05). There was significant difference in plasma CORT between normal control group and liver depression model group, and there was significant difference between three groups (P 0.05). The reliability of the rat model of liver depression induced by chronic restraint stress was verified in two aspects of the difference of neuroendocrine system indexes. ACP and 伪 -Glu in gonad tissue of rats were detected by P0.05A and 伪 -Glu, respectively, and there was no clear relationship between the group with stagnation of liver and the control group. But in the process of modeling, After stimulation with electric stimulation rod, white secretion could be seen in the penile mouth of most rats under resting state, which was in line with the symptoms of white turbid disease syndrome in TCM, and showed the presence of essence stasis to some extent. Conclusion. In this study, by using the methods of chronic stress restraint tube braking and electric stimulation rod stimulation, the rats in the group of liver depression and Jingyu showed the phenomenon of dropping white penis during the period of stimulation, and to some extent showed the presence of fine blood stasis. The relationship between liver depression and blood stasis was explained, and a meaningful attempt was made for further study.
【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332

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