原代培养海马神经元Drebrin和Synaptophysin的表达与动态变化

发布时间：2018-04-02 18:02

本文选题：树突棘　切入点：Drebrin　出处：《神经解剖学杂志》2017年05期

【摘要】：目的:观察原代海马神经元不同时期树突棘的变化以及树突棘蛋白Drebrin和突触囊泡膜蛋白SYN的表达水平。方法:光镜观察原代培养7、14和20 d的海马神经元的形态改变。应用免疫荧光对Drebrin和SYN进行染色,观察树突棘形态和数量的改变。进一步应用Western Blot检测Drebrin和SYN蛋白的表达变化。结果:培养7 d的海马神经元,未见树突棘结构,SYN斑点状阳性产物较少。随着海马神经元培养时间的加长,突起数量逐渐增加,14 d和20 d的树突棘数量和SYN阳性产物均显著增加。Drebrin和SYN蛋白表达水平也随培养时间的加长而显著增加。结论:原代培养的海马神经元在14 d已有树突棘形成,20 d显示出成熟树突棘的形态和数量。Drebrin和SYN的蛋白表达水平反映了树突棘和突触的形成和功能状态。
[Abstract]:Aim: to observe the changes of dendritic spine and the expression of dendritic spinoprotein Drebrin and synaptic vesicular membrane protein (SYN) in primary hippocampal neurons at different stages.Methods: the morphological changes of hippocampal neurons in primary culture were observed under light microscope for 14 d and 20 d.Drebrin and SYN were stained with immunofluorescence to observe the morphological and quantitative changes of dendritic spine.Western Blot was used to detect the expression of Drebrin and SYN protein.Results: in cultured hippocampal neurons for 7 days, there were few SYN spots positive in the structure of dendritic spine.With the increase of hippocampal neuron culture time, the number of dendritic spines and the expression of SYN positive products increased significantly at 14 and 20 days. The expression of .Drebrin and SYN protein also increased with the increase of culture time.Conclusion: the primary cultured hippocampal neurons have been formed dendritic spine for 20 days after 14 days. The protein expression level of Drebrin and SYN reflects the formation and function of dendritic spine and synapse.
【作者单位】：河北医科大学人体解剖学教研室;河北医科大学组织胚胎学教研室;
【基金】：河北省高等学校科学技术研究项目(QN2014016) 河北省博士后科研项目择优资助(B2016003023)
【分类号】：R338

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