β3肾上腺素受体在人子宫平滑肌的表达及意义

发布时间：2018-04-03 00:24

本文选题：β3-AR　切入点：早产　出处：《中南大学》2012年硕士论文

【摘要】：目的探讨β3肾上腺受体(β3-adrenoceptor,p3-AR)在人子宫平滑肌中的表达及与分娩发动的关系。方法选择2011年6月～2012年2月在中南大学湘雅二医院住院妊娠足月未临产产妇15例(未临产组,not in labor,NL组)、足月临产产妇15例(临产组,in labor,L组)、未妊娠因宫颈上皮内瘤样变行子宫全切术妇女15例(未妊娠组,in non-pregnant,NP组)。应用实时荧光定量RT-PCR和免疫印迹(Western Blot)检测3组妇女子宫平滑肌中p3-AR的mRNA和蛋白的表达水平。结果 1、妊娠足月未临产组、足月临产组、未妊娠组均有p3-AR的表达； 2、足月临产组3-AR mRNA的相对表达量(0.317+0.06)明显低于足月未临产组(0．714±0.103),P=0.000,P0.05。足月未临产组表达明显高于未妊娠组(0.282+0.075),P=0.000,P0.05。非妊娠组与足月临产组相比无显著差异,P=-0.25,P0.05。 3、足月临产组D3-AR蛋白的相对表达量(0.379±0.027)明显低于足月未临产组(0.690±0.054),P=0.000,P0.05。足月未临产组蛋白相对表达量明显高于未妊娠组(0.362+0.045),P=0.000,P0.05。未妊娠组与足月临产组相比无显著差异,P=0.3,P0.05。结论1、子宫平滑肌中有p3-AR的表达；2、子宫平滑肌中p3-AR表达减少与分娩发动有关。
[Abstract]:Objective to investigate the expression of 尾 3 adrenoceptor p3 ARR in human uterine smooth muscle and its relationship with labor initiation.Methods from June 2011 to February 2012, 15 pregnant women who were hospitalized in Xiangya No.2 Hospital of Central South University (not in labor group) and 15 women in term delivery group (labor group) were selected.Total hysterectomy was performed in 15 women with tumor-like degeneration (non-pregnant NP group).The expression of p3-AR mRNA and protein in the uterine smooth muscle was detected by real-time fluorescence quantitative RT-PCR and Western blotting.Result1. The expression of p3-AR was found in the full-term labor group, the term labor group and the non-pregnancy group.2, the relative expression of 3-AR mRNA in term labor group (0.317 0.06) was significantly lower than that in term non-labor group (0.714 卤0.103).The expression of P0. 282 0. 075 was significantly higher in term non-parturient group than that in non-pregnant group (P 0. 000 P 0. 05).There was no significant difference between non-pregnancy group and term labor group.The relative expression of D3-AR protein in term labor group (0.379 卤0.027) was significantly lower than that in non-term labor group (0.690 卤0.054).The relative expression of protein in term unpartum group was significantly higher than that in unpregnant group 0.362 0.045% P0. 000 P0. 05.There was no significant difference between non-pregnancy group and term labor group.Conclusion 1. There is p3-AR expression in uterine smooth muscle. The decrease of p3-AR expression in uterine smooth muscle is related to the onset of labor.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R339.2

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