贯穿缝扎联合打孔法构建兔耳难愈性创面模型

发布时间：2018-04-04 18:13

本文选题：兔耳　切入点：难愈性创面模型　出处：《广西医科大学》2012年硕士论文

【摘要】：背景：以往制作的兔耳难愈性创面模型仅局限于单纯的缺血模型,缺血淤血模型少见。目的：建立一种方法简单、效果确实的兔耳难愈性创面模型。方法：健康新西兰大白兔20只即兔耳40个,随机分为缝扎+打孔组(实验组)和单纯打孔组(对照组)两组,每组兔耳20个。实验组贯穿兔耳根部背腹侧皮肤和软骨,间断缝扎中央、头侧所有动、静脉,缝扎后第10天在耳背侧用活检打孔器制作3个深达软骨面、直径6mm的圆形创面。对照组在正常兔耳(血管未行缝扎)背侧用活检打孔器制作与实验组完全相同的3个圆形创面。兔耳缝扎、打孔创面每日外涂0.5%碘伏3次。观察兔耳大体情况、创面愈合时间,打孔后第7、14、21天行苏木精-伊红(hematoxylin-eosin, HE)染色及血管内皮细胞生长因子(vascular endothelial growth factor, VEGF)免疫组织化学染色,分别观察创面肉芽组织生长、表皮化程度及肉芽组织中血管内皮细胞生长因子的表达情况。结果：实验组兔耳缝扎后立即呈现紫黑色,第10、20、30天为淡紫黑色、紫色、淡紫色。实验组、对照组创基肉芽组织生长、表皮化时间分别为第3-4天、第16-18天和第1-2天、第11-13天。实验组与对照组比较,在创面愈合时间(P0.01)、创面肉芽组织生长、表皮化时间均延迟,血管内皮细胞生长因子表达实验组低于对照组(P0.01)。结论：贯穿缝扎联合打孔法制作的兔耳难愈性创面模型方法简单、效果确实可靠,可作为缺血淤血因素所致难愈性创面修复的实验研究模型。
[Abstract]:Background: Previous wound healing of rabbit ear model making limited to simple models of ischemia, a rare model of ischemia and blood stasis.
Objective: to establish a simple method of wound of rabbit ears, refractory model does effect.
Methods: 20 healthy New Zealand white rabbits: 40 rabbits, randomly divided into suture and punch group (experimental group) and simple perforation group (control group) two groups, each group of 20 rabbits. The experimental group through rabbit ear root dorsal ventral skin and cartilage, interrupted suture central, all side head move, vein, suture after tenth days in the ear side with biopsy punch 3 deep cartilage 6mm diameter circular wounds. In the control group of normal rabbit ears (without vascular suture) 3 circular dorsal wound biopsy punch fabrication and experimental group rabbit exactly the same. Ear suture, 3 punch wound daily coated with 0.5% iodophor. Rabbit ears to observe the general situation, the wound healing time, perforation after 7,14,21 days of hematoxylin eosin staining (hematoxylin-eosin, HE) and vascular endothelial growth factor (vascular endothelial, growth factor, VEGF) immunohistochemical staining were observed in the granulation group The growth factor, the degree of epidermis and the expression of vascular endothelial growth factor in the granulation tissue.
Results: the experimental group rabbit ear suture violet black immediately after 10,20,30 days, the purple black, purple, pale purple. The experimental group, the control group was the growth of granulation tissue and epidermal time respectively for the first 3-4 days, the 16-18 day and the 1-2 day, the 11-13 day. The experimental group compared with control group and in wound healing time (P0.01), the growth of granulation tissue and epidermis of time delay, the expression of vascular endothelial growth factor in experimental group than in the control group (P0.01).
Conclusion: the suture of wound healing in rabbit ear model ligation combined punch made of simple, reliable effect, can be used as an experimental model for the study of repair caused by ischemia and congestion factors of refractory wounds.

【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R641;R-332

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