几种色素抑制过氧亚硝酸根损伤酪氨酸能力的对比

发布时间：2018-04-05 03:17

本文选题：高效液相色谱-二极管阵列检测器　切入点：荧光光谱法　出处：《湖南师范大学自然科学学报》2017年06期

【摘要】：过氧亚硝酸根可以通过强氧化性及强硝化性损伤酪氨酸,从而损害人体健康.姜黄素、原花青素、槲皮素和柚皮素具有抗氧化、抗硝化性,但其自身颜色在研究酪氨酸硝化损伤的常规方法中易造成干扰.为测定色素抑制3-硝基酪氨酸生成的能力,使用高效液相色谱-二极管阵列检测器(HPLC-PDAD,High Performance Liquid Chromatography-Photodiode Array Detector)法对硝化损伤体系成分进行分离,使用荧光光谱法测定色素抑制酪氨酸二聚体生成能力,邻苯三酚自氧化法测定超氧根阴离子抑制能力.结果表明3-硝基酪氨酸抑制率从大到小为姜黄素、原花青素、柚皮素、槲皮素、V_C;酪氨酸二聚体抑制率从大到小为原花青素、姜黄素、柚皮素、槲皮素、V_C;邻苯三酚自氧化抑制率从大到小为姜黄素、槲皮素、柚皮素、V_C,原花青素无明显影响.分析表明4种色素成分抗氧化、抗硝化能力与其结构密切相关.
[Abstract]:Peroxynitrite can damage tyrosine through strong oxidation and strong nitrification, thereby harming human health.Curcumin, procyanidin, quercetin and naringin have anti-oxidation and nitrification properties, but their own color is easily interfered in the conventional method of studying tyrosine nitrification damage.In order to determine the ability of pigment to inhibit the formation of 3-nitrotyrosine, high performance liquid chromatography-diode array detector (HPLC-PDADX) high Performance Liquid Chromatography-Photodiode Array detector was used to separate the components of nitrification damage system.The ability of pigment to inhibit tyrosine dimer was measured by fluorescence spectrometry, and the inhibition of superoxide anion was measured by pyrogallol autooxidation.The results showed that the inhibition rate of 3-nitrotyrosine was curcumin, proanthocyanidin, naringin, quercetin, and tyrosine dimer from large to small to proanthocyanidin, curcumin, naringin,The inhibition rates of pyrogallol autooxidation were curcumin, quercetin, naringin and procyanidins.The results showed that the antioxidant and nitrification resistance of the four pigment components were closely related to their structure.
【作者单位】：北京工业大学生命科学与生物工程学院;
【基金】：国家科技支撑计划资助项目(2015BAK44B00)
【分类号】：TS264.4

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