麝香对颅骨骨缺损模型大鼠SCF和MCP-1 mRNA表达的影响及意义

发布时间：2018-04-06 05:00

本文选题：干细胞因子　切入点：单核细胞趋化蛋白　出处：《中国骨质疏松杂志》2017年03期

【摘要】：目的分析干细胞因子(SCF)、单核细胞趋化蛋白1(MCP-1)在颅骨骨缺损模型大鼠中的作用及麝香对二者表达的影响。方法通过颅骨钻建立颅骨骨缺损模型,将模型大鼠随机分为给药组与模型组,再将给药组和模型组随机分为3小组,给药组灌服麝香,模型组灌服生理盐水,分别在干预后7 d、14 d、28 d通过酶联免疫吸附法(ELISA)检测各组大鼠血清中SCF表达及采用实时荧光定量PCR法检测颅骨骨缺损处MCP-1 mRNA含量的表达变化。结果与模型组比较,给药组第14天SCF表达最低,且无显著性,第7天和第28天其表达均比第14天升高,这两时间段与模型组比较差异均有统计学意义(P=0.0002、0.0025),但是第7天SCF表达增加更加显著;给药组在第7天MCP-1 mRNA表达水平最高,与模型组比较差异有统计学意义(P0.05),第14天和第28天其表达持续降低,与模型组比较差异均无统计学意义(P=0.0633、0.1597)。结论SCF、MCP-1在颅骨骨缺损修复愈合过程中可能起重要作用,麝香通过增加模型大鼠血清中SCF的表达和颅骨骨缺损组织MCP-1 mRNA的表达,间接反映出骨缺损修复愈合机制可能与增加SCF和MCP-1 mRNA表达有关,麝香在加速骨缺损修复与愈合微环境中起着重要作用。
[Abstract]:Objective to investigate the role of stem cell factor (SCF), monocyte chemoattractant protein 1 (MCP-1) in cranial bone defect rats and the effect of musk on their expression.Methods the model of skull bone defect was established by skull drill. The model rats were randomly divided into two groups: the administration group and the model group. Then the drug group and the model group were randomly divided into 3 groups: the drug group was given musk and the model group was given normal saline.The expression of SCF in serum of rats in each group was detected by enzyme-linked immunosorbent assay (Elisa) and the expression of MCP-1 mRNA in the skull defect was detected by real-time fluorescence quantitative PCR.Results compared with the model group, the expression of SCF in the treatment group was the lowest on the 14th day and had no significant difference. The expression of SCF on the 7th and 28th day was higher than that on the 14th day.The difference between the two groups was statistically significant, but the expression of SCF increased more significantly on the 7th day, and the expression of MCP-1 mRNA was the highest in the treatment group on the 7th day.Compared with the model group, the expression of P0. 05 was significantly decreased on the 14th and 28th day, but there was no significant difference between the model group and the model group.Conclusion SCF- MCP-1 may play an important role in the repair and healing of skull defect. Musk can increase the expression of SCF in serum and MCP-1 mRNA in skull-bone defect tissue.It is suggested that the mechanism of bone defect healing may be related to the increased expression of SCF and MCP-1 mRNA. Musk plays an important role in accelerating the repair and healing of bone defect in microenvironment.
【作者单位】：甘肃中医药大学;甘肃省中医院;
【基金】：国家自然科学基金项目(81460735) 国家中医药管理局国家中医临床研究基地业务建设科研专项(JDZX2015080)
【分类号】：R285.5;R-332

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