丹参通络解毒汤对骨髓干细胞移植急性心肌梗死模型大鼠NF-κB介导的炎症反应影响

发布时间：2018-04-07 16:35

本文选题：丹参通络解毒汤　切入点：急性心肌梗死　出处：《中国中医基础医学杂志》2017年07期

【摘要】：目的:探讨丹参通络解毒汤对骨髓干细胞(BMSCs)移植急性心肌梗死(AMI)模型大鼠核因子-κB(NF-κB)介导炎症反应的影响。方法:80只大鼠按随机数字表法分为SH组(假手术组)、AMI组(模型组)、BMSCs组(BMSCs移植组)、DSTLJD组(丹参通络解毒汤组)、DSTLJD+BMSCs组(丹参通络解毒汤+BMSCs移植)各10只,BMSCs细胞悬液直接注入梗死区边缘心肌组织,给予中药或生理盐水灌胃,4周后采用酶联免疫吸附法(ELISA)测定各组大鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的含量,免疫组化法观察心肌核因子-κB(NF-κB)蛋白表达,并用HE染色观察心肌组织形态病理变化。结果:与AMI组比较,BMSCs组、DSTLJD组、DSTLJD+BMSCs组大鼠心肌NF-κB蛋白表达明显降低,且血清炎症因子TNF-α和IL-6水平明显下降,差异有统计学意义,心肌损伤程度明显改善;DSTLJD+BMSCs组优于单纯BMSCs组、单纯DSTLJD组,差异有统计学意义。结论:BMSCs移植联合丹参通络解毒汤能够改善AMI模型大鼠的心肌损伤,减轻炎症反应,修复缺血心肌,其机制可能与调节NF-κB介导的炎症反应有关。
[Abstract]:Aim: to investigate the effect of Danshen Tongluo jiedu decoction on inflammatory response mediated by nuclear factor-魏 B (NF-魏 B)-魏 B in acute myocardial infarction (AMI) rats transplanted with bone marrow stem cells (BMSCs).Methods: 80 rats were randomly divided into SH group (sham operation group) and AMI group (model group, BMSCs group) and DSTLJD group (Danshen Tongluo jiedu decoction group, DSTLJD BMSCs group (Danshen Tongluo jiedu decoction BMSCs transplantation))Into the myocardial tissue at the edge of the infarct area,Serum TNF- 伪 TNF- 伪 (IL-6) and myocardial nuclear factor- 魏 B (NF- 魏 NF- 魏 B) protein expression were measured by Elisa after 4 weeks of oral administration of traditional Chinese medicine or normal saline.The histopathological changes of myocardium were observed by HE staining.Results: compared with AMI group, the expression of NF- 魏 B protein and the levels of serum inflammatory factor TNF- 伪 and IL-6 in DSTLJD BMSCs group were significantly decreased, and the degree of myocardial injury was significantly improved in DSTLJD BMSCs group than in BMSCs group.The difference was statistically significant in simple DSTLJD group.Conclusion the transplantation of 10% BMSCs combined with Danshen Tongluo jiedu decoction can ameliorate myocardial injury, alleviate inflammatory reaction and repair ischemic myocardium in AMI rats. The mechanism may be related to the regulation of NF- 魏 B mediated inflammation.
【作者单位】：湖南中医药大学中医学院;
【基金】：湖南省教育厅科学研究重点项目(15A143) 湖南省中医药科研计划项目(201559) 国家中医药管理局重点学科“中医各家学说”开放基金项目(2014GJXS02)
【分类号】：R285.5;R-332

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