山茱萸多糖对阿尔茨海默病模型大鼠认知能力和海马磷酸化-tau蛋白表达的影响

发布时间：2018-04-08 09:49

本文选题：山茱萸多糖　切入点：阿尔茨海默病　出处：《中国老年学杂志》2017年13期

【摘要】：目的观察山茱萸多糖对阿尔茨海默病(AD)大鼠认知能力的影响及作用机制。方法 100只Wistar大鼠随机分为青年组、假手术组、模型组、多奈哌齐组、山茱萸多糖组各20只。采用D-半乳糖联合β-淀粉样蛋白(Aβ)1~40注射建立AD模型。水迷宫实验观察大鼠认知能力,免疫组化(SABC)法检测大鼠海马CA4区磷酸化tau(p-tau)蛋白表达量。结果药物干预后,山茱萸多糖组平均逃避潜伏期较模型组明显缩短(P0.05);120 s穿越原平台次数较模型组明显增加(P0.05)。山茱萸多糖组海马区p-tau蛋白阳性染色较模型组明显减弱(P0.05)。结论山茱萸多糖对AD大鼠认知能力有改善,可能与降低海马区p-tau蛋白表达量相关。
[Abstract]:Objective to observe the effect of Fructus Corni polysaccharides on cognitive ability and its mechanism in rats with Alzheimer's disease (AD).Methods 100 Wistar rats were randomly divided into youth group, sham operation group, model group, Donepezil group and Cornus officinalis polysaccharide group.The AD model was established by injection of D-galactose and 尾 -amyloid A 尾 -Amyloid 40.The cognitive ability of rats was observed by water maze test, and the expression of phosphorylated tau-tau-tau-taup protein in CA4 region of hippocampus was detected by immunohistochemical staining.Results after drug intervention, the average escape latency of Cornus officinalis polysaccharide group was significantly shorter than that of the model group.The positive staining of p-tau protein in hippocampus of Cornus officinalis polysaccharide group was significantly lower than that of model group.Conclusion Fructus Corni polysaccharides can improve the cognitive ability of AD rats, which may be related to the decrease of p-tau protein expression in hippocampus.
【作者单位】：佳木斯大学附属第一医院;佳木斯大学基础医学院;
【基金】：黑龙江省卫生厅项目(2013-233) 佳木斯大学面上项目(L2012-052) 佳木斯大学附属第一医院培育课题(jy2014-005)1佳木斯大学基础医学院
【分类号】：R285.5;R-332

【相似文献】