裸鼠盲肠黏膜下注射HCT116细胞建立结肠癌原位种植及转移模型的研究

发布时间：2018-04-11 05:02

本文选题：人结肠癌HCT细胞株 + 原位种植癌　；参考：《北京中医药大学学报》2017年10期

【摘要】：目的建立一种简便、可行、稳定的裸鼠盲肠黏膜原位种植癌及转移模型。方法将人结肠癌细胞株HCT116细胞注射接种于裸鼠盲肠黏膜下,HE染色及人癌胚抗原(CEA)免疫组化检测接种后不同时期的盲肠原位瘤,腹腔淋巴结、肝、肺组织转移情况。结果盲肠接种HCT116细胞后2周,90%裸鼠均长出直径2~5 mm的盲肠原位瘤;接种后4周开始有腹腔淋巴结转移,12周时淋巴结转移率100%;接种10周时见肝、肺有转移,12周时肝、肺转移率分别为60%、39%。结论利用盲肠黏膜下原位接种HCT116细胞法建立裸鼠结肠癌模型,可模拟结肠癌临床原位发生、发展演变过程,方法简便且成瘤率高,为深入研究结肠癌转移机制的基础研究提供理想、稳定的动物模型。
[Abstract]:Objective to establish a simple, feasible and stable model of in situ implanted carcinoma and metastasis of caecal mucosa in nude mice.Methods Human colon cancer cell line HCT116 cells were injected into the caecum submucosa of nude mice by HE staining and human carcinoembryonic antigen (CEA) immunohistochemical staining to detect the metastasis of cecal in situ tumor, abdominal lymph node, liver and lung tissues at different stages after inoculation.Results at 2 weeks after inoculation of HCT116 cells, 90% of the nude mice grew caecum in situ tumor with a diameter of 2 ~ 5 mm, the rate of lymph node metastasis was 100% at 12 weeks after inoculation, liver was found at 10 weeks after inoculation, and liver was found in lung at 12 weeks after inoculation.The lung metastasis rate was 60% and 39% respectively.Conclusion the model of colon cancer in nude mice can be established by in situ inoculation of HCT116 cells in subcecal mucosa, which can mimic the clinical in situ occurrence and evolution of colon cancer. The method is simple and has a high rate of tumorigenesis.To provide an ideal and stable animal model for further study on the mechanism of colon cancer metastasis.
【作者单位】：湖南中医药大学;湖南省中医药研究院附属医院;
【基金】：国家自然科学基金面上项目(No.81373602) 湖南中医药大学在读研究生项目(No.2016CX06)~~
【分类号】：R-332;R735.35

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